香叶木苷在大鼠体内的药代动力学研究  

Studies on pharmacokinetics of diosmin in rats

作　　者：马英丽[1] 马超[1] 

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《中国中药杂志》2007年第5期418-420,共3页China Journal of Chinese Materia Medica

基　　金：黑龙江省科技攻关计划项目(GC03C402-1)

摘　　要：目的：建立香叶木苷血药浓度的高效液相色谱测定方法，并探讨其在大鼠体内的药代动力学特点。方法：大鼠灌胃香叶木苷225，325，425mg·kg^-1后，于不同时间点采血，用HPLC测定其血药浓度并用3p97软件拟合，求算药动学参数。结果：香叶木苷血药浓度的回归方程为Y=3．05×10^-3C＋1．55×10^-3，R=0．9964，在0．5—100μg·mL^-1线性关系良好，最低检测限为0．2μg·mL^-1。方法回收率大于92％。大鼠灌胃香叶木苷以高，中，低3个剂量后，其体内过程经3p97拟合符合一室模型。结论：首次建立了香叶木苷血药浓度的HPLC检测方法，该方法专属性强，灵敏度高，可用于该药的体内定量分析。Objective： To establish a HPLC method for the determination of diosmin in Bats plasma and to study the pharmacokinetics of diosmin in Bats. Method： Rats were given diosmin with 3 doses as 225,325,425 mg · kg^- 1. Blood samples were collected at different times after oral administration. The plasma concentration of diesmin was determined by HPLC, and the pharmacokineties parameters were calculated by 3p97 program. Result： The typical equation of diosmin in rats plasma was Y = 3.05 × 10^-3 C ＋ 1.55 × 10^-3, the calibration curves of diosmin was linear in the range from 0. 5-100 μg · mL^-1 （R =0. 996 4）. The lowest concentration of diosmin in plasma was 0. 2 g · mL^-1. Its recoveries was more than 85%, and the interday and intraday precision, which was expressed as BSD, were all less than 15%. After 3 doses oral administration of diosmin in rats, the mean plasma concentration-time curves were found to fit one compartment model, and the main phannacokinetics parameters were obtained. Conclusion： It is first time to establish the HPLC method to determine the concentration of diosmin in rats plasma, and the method described in this report has high sensitivity and selectivity, and it was suitable for phannacokinetics studies of diosmin. The internal process of diosmin in rats is fit to one comportment model.

关 键 词：香叶木苷 HPLC 药代动力学 一室模型 

分 类 号：R285.5[医药卫生—中药学]