铜(Ⅱ)-天冬氨酸-咪唑类配合物的合成及其与DNA作用的光谱研究与比较  

Synthesis and DNA Binding Spectroscopic Studies of Cu(Ⅱ)-HAsp-Im Complexes

作　　者：张芳[1] 张前前[1] 陆小兰[1] 江涛[1] 王修林[1] 

机构地区：[1]中国海洋大学化学化工学院,山东青岛266003

出　　处：《光谱学与光谱分析》2007年第2期302-305,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20371043)资助

摘　　要：合成了两种新的铜(Ⅱ)-天冬氨酸-咪唑类混配配合物([Cu(HAsp)ImH2O]SO4.4H2O与[Cu(Asp)Im(OH)].4H2O,HAsp代表天冬氨酸分子,Asp代表天冬氨酸离子,Im代表咪唑)。以元素分析、红外光谱及热重-差热分析对其进行表征;以电子吸收光谱法及荧光分析法研究了这两种配合物与DNA的作用。结果表明两种铜(Ⅱ)-天冬氨酸-咪唑类混配配合物与DNA的作用方式明显不同[Cu(HAsp)ImH2O]SO4.4H2O为伴随着静电作用的插入结合;而[Cu(Asp)Im(OH)].4H2O主要与DNA的碱基N发生配位作用,造成了DNA双螺旋的破坏。分析了两种配合物因结构不同而导致的与DNA作用方式不同的原因。Two novel complexes of [Cu（HAsp） ImH2O] SO4 · 4H2O and [Cu （Asp） Im （OH）] · 4H2O （ HAsp = Aspartic acid molecule, Asp=Aspartic acid ion, Im= Imidazole） were synthesized and characterized by elemental analysis, IR spectroscopy and TG-DTA. The interactions of the complexes with sperm DNA were studied by electronic absorption and fluorescence spectra. Results showed that the reactions of the two complexes with DNA are obviously different： for [Cu（HAsp）ImH2O]SO4·4H2O, it is intercalation companied by electrostatic effect, while [Cu（Asp）Im（OH）] · 4H2 O mainly cooperates with the nitrogen atom of the DNA base pair, which induces the breakage of DNA double helix. The reasons for these differences in their DNA binding modes were also discussed.

关 键 词：铜(Ⅱ)-天冬氨酸-咪唑类混配配合物 鱼精DNA 作用模式 结构影响 

分 类 号：O614.1[理学—无机化学]