高分子量昆虫病毒DNA基因组的电镜观察——Ⅰ关于解旋高度致密的超螺旋结构的尝试  

Electron Microscopic Observation of High Molecular Weight DNA Genome

作　　者：李维琪[1] 杨向明[1] 唐桂芳[1] 吾尔尼沙[1] 

机构地区：[1]中国科学院新疆化学研究所

出　　处：《电子显微学报》1989年第2期1-5,共5页Journal of Chinese Electron Microscopy Society

摘　　要：本文介绍一种解旋高度致密的超螺旋结构得到完全开环分子图像的技术,与一些常规方法相比,此法有独特之处。这一技术为用电镜精确测定高分子量基因组的分子量、进行基因定位及进行复制转录的研究提供了方便。Insect baculovirus have high molecular weight DNA genomes with about 50-100×daldons. In rutine methods micrographs of highly supercoiled molecules have been obtained. Using a new method, incubation with SDS following gently stirring, pbotograpns of completely opened circular molecules were gained succesfuly. It will be convenient to demonstrate the location of certain genes on the genome and the physicai location for RNA transcription site.

关 键 词：电镜 DNA 昆虫 螺旋结构 病毒 

分 类 号：Q523.4[生物学—生物化学]