低温胁迫诱导灰木相思组培苗细胞凋亡  

Apoptosis induced by cold stress in plantlets of Acacia implexa

作　　者：何文锦[1] 代容春[2] 连玲[2] 陈由强[2] 林思祖[1] 

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建师范大学生命科学学院,福建福州350007

出　　处：《福建农林大学学报（自然科学版）》2007年第1期38-42,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省自然科学基金资助项目(B0510009);福建省林业厅种苗科技攻关项目(闽林综[2003]17号)

摘　　要：利用显微观察和DNA凝胶电泳等技术,研究在低温胁迫诱导下灰木相思组培苗细胞的凋亡和对低温环境的耐受性.显微结构观察表明,随着温度降低,细胞核出现明显的凋亡特征,证实低温胁迫可诱导灰木相思组培苗细胞凋亡,同时灰木相思组培苗对低温的耐受性是有限的,超过某一强度时,细胞会死亡.Apoptosis and the tolerance of plantlets of Acacia implexa treated in vitro with cold stress were studied by microphotographics and total DNA agarose gel electrophoresis. It was found that the apoptosis characteristic of nucleus appeared while lowering the temperature. The results showed that cold stress caused apoptosis of the planflets of A. implexa in vitro which had a limitation in the tolerance to cold stress.

关 键 词：低温胁迫 灰木相思 组培苗 细胞凋亡 

分 类 号：Q942.5[生物学—植物学]