稻瘟菌若干T-DNA插入突变体初步分析  

Analysis of Magnaporthe grisea mutants through T-DNA insertion

作　　者：魏艺聪[1] 潘初沂[1] 李宏宇[1] 鲁国东[1] 雷财林[2] 王宗华[1] 

机构地区：[1]福建农林大学功能基因组学研究中心,福建福州350002 [2]中国农业科学院重大工程中心,北京100081

出　　处：《福建农林大学学报（自然科学版）》2007年第1期47-50,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30370924);福建省自然科学基金资助项目(B0520002).

摘　　要：为揭示稻瘟菌致病分子机制,创建了该菌T-DNA插入突变体库．部分突变体经表型分析,获得3个生长发育及致病性同时发生变异,5个生长发育正常但致病性变异及2个生长发育变异但致病性正常的突变体；以TAIL-PCR方法获得了这些突变体T-DNA插入位点及其边界序列,并用生物信息方法对被T-DNA标签的基因进行了分析,为进一步研究这些基因的功能提供了重要信息．To explore molecular mechanism in regulating pathogenesis of the rice blast fungus, Magnaporthe grisea, a T-DNA insertional mutant library of the fungus was constructed. Partial mutants in the library were selected to conduct phenotype and genetics characterization. Among these, 3 mutants showed mutation both in growth and pathogenicity, 5 showed normal in growth but mutated in pathogenecity, while other 2 showed mutated in growth but normal in pathogenecity. The T-DNA flanking region of these mutants was further amplified by TAIL-PCR. The T-DNA target genes were applied to analyze by bioinformatics methods. These data will provide us important information to further study the function of these genes.

关 键 词：稻瘟菌 T-DNA插入变异 生物信息 

分 类 号：Q751[生物学—分子生物学]