大鼠胫骨骨折愈合过程中Ⅰ、Ⅱ型胶原蛋白的表达:失神经状态下Western blot方法测定  

Expressions of type Ⅰ and type Ⅱ collagen detected by Western blot analysis during tibial fracture healing in denervate rats

作　　者：马骋[1] 苟三怀[2] 何仿[2] 肖海军[2] 

机构地区：[1]北华大学附属医院骨一科,吉林省吉林市132001 [2]解放军第二军医大学长征医院骨科,上海市200006

出　　处：《中国组织工程研究与临床康复》2007年第10期1854-1857,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:揭示失神经骨折愈合中Ⅰ、Ⅱ型胶原蛋白表达的变化规律,探讨神经系统对骨折愈合机制的影响。方法:实验于2005-05/12在解放军第二军医大学动物实验室及细胞生物教研究室完成。40只SD大鼠按随机数字表法分成2组,每组20只。单纯左胫骨骨折组(胫骨骨折组),于左胫骨前弓状缘上0.3cm上1/3处咬断胫骨,从胫骨平台前缘插入一根直径0.8mm克氏针,制成胫骨骨折模型。T10脊髓完全性损伤并左胫骨骨折组(脊髓损伤并胫骨骨折组),在上述模型的基础上,横断切除T10段脊髓约0.3cm,制成T10脊髓完全性损伤大鼠胫骨骨折模型制备。分别于伤后1,2,4,5周采用Westernblot方法检测骨痂中Ⅰ、Ⅱ型胶原的蛋白表达。结果:40只大鼠全部进入结果分析。伤后第1周,两组Ⅰ、Ⅱ型胶原均有表达,脊髓损伤并胫骨骨折组两种胶原的表达程度均高于胫骨骨折组;伤后第2周,脊髓损伤并胫骨骨折组Ⅱ型胶原的表达达到峰值,高于胫骨骨折组(39.45±0.99,29.45±0.85,aP<0.05);伤后第4周,胫骨骨折组Ⅰ型胶原表达量达峰值,高于脊髓损伤并胫骨骨折组(31.69±1.42,26.33±1.11,aP<0.05),脊髓损伤并胫骨骨折组Ⅱ型胶原仍有高表达;伤后第5周,胫骨骨折组、脊髓损伤并胫骨骨折组Ⅰ、Ⅱ型胶原表达下降(Ⅰ型胶原2组依次为:20.32±0.56,13.57±0.44;Ⅱ型胶原2组依次为6.34±0.21,12.58±0.44)。结论:神经系统可能通过调节Ⅰ、Ⅱ型胶原的分泌而影响骨折愈合。AIM： To disclose the Change rules of type Ⅰ and Ⅱ collagen protein during denervate fractures healing in rats and investigate the influence of nervous system on the mechanism of fracture healing. METHODS： The experiment was conducted in the animal laboratory and cell biological laboratory, Second Military Medical University of Chinese PLA from May to December 2005. Forty SD rats were randomly assigned into two groups with 20 rats in each group： Simple left tibial fractures group （F group）： The upper 1/3 of 0.3 cm of the left tibial arcuate border was bite off and one 0.8 mm in diameter Kirschner wire was inserted from the anterior border of tibial plateau to establish the tibial fracture models; Tlo spinal cord complete injury combined left tibial fracture group （FS group）： Based on the tibial fracture models, 0.3 cm T10 spinal cord was tranSected and removed to establish the FS models. The expressions of type Ⅰ, Ⅱ collagen ware observed with Westem blot at 1, 2, 4 and 5 weeks postoperatively. RESULTS： All 40 rats ware involved in the result analysis. The expression of both collagens were found in the two groups at 1 week, and the FS group was higher than the F group; at 2 weeks, the expressions of type Ⅱ collagen in the FS group reached the peak value, and significantly higher than the F group （39.45±0.99, 29.45±0.85, ap 〈 0.05）; the peak expression of type I collagen was found in F group at the forth week, and higher than the FS group （31.69±1.42, 26.33±1.11, aP 〈 0.05）, but the expression of type.Ⅱcollagen was still obvious in the FS group; the expressions of F and FS groups ware reduced obviously at the 5^th week （type Ⅰ collagen： 20.32±0.56, 13.57±0.44; type IT collagen： 6.34±0.21, 12.58±0.44）. CONCLUSION： The nervous system influences the fracture healing by regulating the production of type Ⅰ and Ⅱ collagens.

关 键 词：去神经支配 骨折愈合 胶原Ⅰ型 胶原Ⅱ型 

分 类 号：R683[医药卫生—骨科学]