冷冻异种骨膜引导骨组织的再生  

Frozen heterogeneous periosteum can guide bone tissue regeneration

作　　者：刘希云[1] 张德辉[1] 张惠[1] 周丛豪[1] 刘晓勇[2] 

机构地区：[1]北京世纪坛医院口腔科,北京市100038 [2]北京口腔医院病理科,北京市100050

出　　处：《中国组织工程研究与临床康复》2007年第10期1969-1971,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:探讨应用冷冻异种骨膜作为引导骨组织再生膜的可能性。方法:实验于2002-03/12在北京世纪坛医院动物实验室完成。①冷冻异种骨膜的制备:取新鲜猪头颅顶部骨膜或肋骨骨膜以生理盐水反复漂洗,拭干置于体积分数为0.75的乙醇中浸泡脱脂,然后放置于液态氮中冷冻10min,反复3次后,用γ射线辐照3kGy,储存于低温冰箱内备用。②动物实验:在30只日本大耳白兔下颌骨造成贯穿骨缺损两处,一孔表面覆盖经处理的骨膜为实验组,另一孔不覆盖为对照组。③指标观察:分别在2,4,8,12,16周处死兔,进行大体、X射线片及组织学观察。结果:30只白兔全部进入结果分析。①实验组及对照组大体标本观察:8周,可见引导膜,膜边界已不整齐。实验组平坦,骨质较硬。对照组愈合,但骨质较软;12周,引导膜与周围界限不清,但仍可见。实验组平坦,骨质较硬。对照组骨质较软,仍可见凹陷。②组织病理学观察结果:8周实验组缺损处为新生骨边缘变致密,移植膜内炎症细胞消失,膜内纤维开始水肿,断裂;12周,实验组骨小梁粗大,排列整齐。对照组缺损处成骨量较少,骨小梁比实验组细小。移植膜内无明显炎症,移植膜与周围组织界限不清。③X射线片观察:4,8周实验组明显较对照组密度增高。12,16周实验组基本与周围骨质相似,对照组仍显密度较低。结论:经冷冻处理的异种骨膜在体内不产生明显的排斥反应,可在体内维持8￣12周,具有较好的阻挡纤维组织长入创面、分隔不同细胞及引导组织再生的功效,是一种可以利用的引导组织再生膜材料。AIM： To explore the feasibility of bone tissue regeneration guidance with the frozen heterogeneous pedosteum. METHODS. The experiment was conducted in the animal laboratory of Beijing Shijitan Hospital from March to December 2002. ① Preparation of the frozen heterogeneous pedosteum： The fresh pigs＇ cranial or db bone pedosteum was taken and washed with saline solution repeatedly, then defatted by immersion in 0.75 volume fraction alcohol, followed by frozen in the liquor of nitrogen for 10 minutes repeatedly for 3 times. After irradiated under .y-ray up to the amount of 3 kGy, the created membrane was placed in refrigerator for using. ②Surgical procedure; Two artificial mandibular bone defects ware performed in 30 adult Japanese white big ear rabbits. One defect was covered with the created membrane as the expedmental group, while another as the control group. ③ Index observation： The rabbits ware sacrificed after 2, 4, 8, 12, 16 weeks postoperatively for macroscopic, radiographic and histopatholegical observation. RESULTS： All 30 rabbits ware involved in the result analysis. ① Macroscopic examination of the two groups： At the eighth week, guiding membrane was observed with irregular border. The expedment group was fiat and stiff, while the control group was restored but with soft bone. at the twelfth week, the guiding membrane and surrounding border ware unclear, but still could be observed. The newly bone of the expedment group was fiat and stiff, while the control group was soft with hollow. ② Histopathological examination： At the eighth week, the new-formed bone in the margin of defect area of the expedmental group grew intensively and the inflammatory cells in grafted membrane disappeared; intramembrane fiber was found oedema and broken; at the twelfth week, the trabecular bone of the expedmental group was thick and arranged regularly. However, in the control group, the newly formed bone was little in the defect, and the trabecular bone was thinner compared with the expedmental

关 键 词：移植 异种 骨膜 引导组织再生 

分 类 号：R687.2[医药卫生—骨科学]