高效液相色谱法测定血浆内咪达唑仑浓度  

RP-HPLC Determination of Midazolam in Plasma

作　　者：孙海莺[1] 孙忠实[1] 

机构地区：[1]海军总医院

出　　处：《药物分析杂志》1996年第4期222-225,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：本文建立了测定血浆中咪达唑仑浓度的反相高效液相色谱法。样品用缓冲液碱化后加氯仿提取。水浴挥干，加甲醇溶解进样。以Ｃ１８化学键合硅胶为固定相，甲醇—水—乙腈（４７∶３８∶１５）为流动相，安定为内标，在２２０ｎｍ波长定量检测，在５～５００ｎｇ／ｍｌ浓度范围内线性关系良好，最低检测限为３ｎｇ／ｍｌ，方法重现性好，平均回收率为１０１．７６％。用本方法测定７例单剂量输注０．１２ｍｇ／ｋｇ咪达唑仑青年男性志愿者的血药浓度。A reversed-phase high performance liquid chromatographic method for the determination of midazolam in plasma is described in this paper. Diazepam was used as the internal standard. Midazolam was extracted from alkalized plasma with chloroform and evaporated to dryness. HPLC analysis was carried out onμBondpak C18 column with methanol-water-acetonitrile as the mobile phase and UV detection at 220nm. The lower limit of detection of midazolam in plasma is 3ng/ml, calibration curves is linear over the concentration range of 5￣500ng/ml. The assay procedure has been applied to monitoring plasma levels in intravenous infusion in 7 volunteers. The results show that the method is suitable for clinical pharmacokinetic research.

关 键 词：咪达唑仑 高效液相色谱法 血药浓度 监测 

分 类 号：R446.112[医药卫生—诊断学]