环境因子对转hGM-CSF基因鱼腥藻生长与光合的调节  

Regulations of Environmental Factors on Growth and Photosynthesis of Transgenic Anabaena sp.Strain PCC 7120 Harboring hGM-CSF gene

作　　者：魏兰珍[1] 康升云[1] 严君君[1] 马为民[1] 王全喜[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《西北植物学报》2007年第1期193-196,共4页Acta Botanica Boreali-Occidentalia Sinica

基　　金：上海市高等学校科技基金重点项目(05ZZ15);上海市高校选拔培养优秀青年教师科研专项基金项目(RE569)

摘　　要：以转hGM-CSF基因鱼腥藻7120为对象,分别探讨了温度、pH、光强等环境因子和外加碳、氮等基本营养源对转基因藻生长与光合活性的影响.结果表明:当基本环境因子为30℃、pH9.5和90μmol·m-2·s-1光强时,转基因藻生长最快.添加10mmol·L-1的NaHCO3或5g·L-1的葡萄糖对转基因藻的光合活性促进最大;但外加氮源却抑制了转基因藻的光合活性.Cyanobacteria are autotrophic prokaryotes performing oxygenic photosynthesis, and their photosynthetic activity is restricted by various environmental factors. We investigated the effects of temperature, pH and light intensity on the growth of transgenic Anabaena sp. strain PCC 7120 harboring hGM-CSF gene in this research. The results showed that the optimal temperature,pH and light intensity for the growth of transgenic cells were about 30℃ ,9.5 and 90/μmol · m^-2 · s^-1 ,respectively. The changes in photosynthetic activity while transgenic cells were supplemented with carbon or nitrogen source, showed that the photosynthetic activity of transgenic cells was enhanced by adding both inorganic and organic sources. The transgenic cells of Anabaena sp. strain PCC 7120 was optimal to grow in BG-11 medium containing 10 mmol· L^-1 NaHCO3 or 5 g · L^-1 glucose. By contrast,the photosynthetic activity of transgenic cells was inhibited by adding nitrogen source.

关 键 词：鱼腥藻7120 转基因蓝藻 hGM-CSF基因 环境因子 光合活性 

分 类 号：Q948.12[生物学—植物学]