UV-B辐射对烟草光合色素和几种酶的影响  

Effects of Enhanced UV-B Radiation on Photosynthetic Pigments and Some Enzymes in Tobacco

作　　者：刘敏[1] 李荣贵[1] 范海[2] 杜桂彩[1] 

机构地区：[1]青岛大学山东省天然色素重点实验室,山东青岛266071 [2]山东师范大学逆境植物研究所,济南250014

出　　处：《西北植物学报》2007年第2期291-296,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：青岛市自然科学基金项目(05-1-JC-91);山东省优秀中青年科学家奖励基金项目(2002)

摘　　要：对UV-B胁迫条件下烟草叶片相关生理生化指标的变化进行研究.结果表明,UV-B辐照后,烟草叶片内光合色素(叶绿素a、叶绿素b、类胡萝卜素)的含量均呈上升趋势,3种色素比对照分别上升了21%、10%、27%;可溶性蛋白含量呈先上升后下降趋势;过氧化物酶活性明显上升,同工酶图谱分析证实,有一种新的过氧化物酶同工酶表达;利用重组噬夏孢欧文氏菌(Erwinia uredovora)GGPP合成酶免疫家兔,制备了兔抗GGPP合成酶的抗血清.Western blot检测显示,UV-B辐照可使烟草叶片GGPP合成酶(GGPS)的表达量显著提高.Effects of enhanced UV-B radiation on physiological and biochemical characters of tobacco were investigated. The results showed that the photosynthetic pigments （chlorophyll a,cblorophyll b and carotenoids） increased clearly in tobacco leaves under enhanced UV-B radiation compared with which in leaves growing under natural light. The three pigments increased 21 %, 10% and 27 %, respectively. The content of soluble protein increased first and decreased later. The activity of peroxidase increased distinctly at the same time,and a new peroxidase isozyme appeared through analyzing the electropboresis diagram of peroxidase isozymes. The expression quantity of GGPs increased significantly through Western blot analysis.

关 键 词：烟草 UV-B 光合色素 蛋白 过氧化物酶 GGPP合成酶 

分 类 号：Q945.78[生物学—植物学]