用固相萃取法提取净化生物检材中拟除虫菊酯类杀虫剂的实验研究  

EXPERIMENTAL SYUDY ON ISOLATION AND CLEAN-UP SYNTHETIC PYRETHROID INSECTICIDES FROM BIOLOGICAL SAMPLES WITH SOLID PHASE EXTRACTION

作　　者：刘俊亭[1] 范垂昌[1] 

机构地区：[1]中国医科大学法医化学教研室,沈阳110001

出　　处：《中国法医学杂志》1996年第2期92-95,共4页Chinese Journal of Forensic Medicine

摘　　要：固相萃取法（Sep－PakC18小柱）同时萃取净化生物检材（血浆、尿）中的甲醚菊酯、灭扫利、功夫、二氯苯醚菊酯、安绿宝、速灭杀丁和敌杀死。通过气相色谱氢焰检测器、非极性熔融石英大口径毛细管柱及程序升温色谱条件，得到较好的系统分离，并测定萃取回收率在81％～93％（血浆）和90％～102％之间（尿）。实验证明，用70％甲醇稀释检材并用氯仿洗脱可得到较完全的吸附和最佳回收率。A method for simultaneous isolation and clean-up pyrethroid insecticides (methothrin, fenpropathrin,cyhalothrin, permethrin, cypermethrin, fenvalerate and deltamethrin)with a solid phase extractionmethod (Sep-Pak C18 cartridge) from biological samples (human plasma and urine) is presented.The systematic separation of the insecticides was excellently performed by wide-bore capillary gaschromatography with flame ionization detection and temperature programming. The recoveries werebetween 81% and 93% for plasma, and 90% and 102% for urine. Mixing the samples with 70%methanol and eluting with chloroform demonstrated excellent adsorption on C18 and the best recoveries,respectively.

关 键 词：拟除虫菊酯 固相萃取法 气相色谱法 血浆 尿 

分 类 号：R446.11[医药卫生—诊断学]