小麦HMW-GS基因及其AS-PCR分子标记研究进展  

Researches on HMW-GS Genes and Their AS-PCR Molecular Markers in Wheat

作　　者：孙宪印[1] 吴科 钱兆国 米勇 李斯深[1] 

机构地区：[1]山东农业大学农学院,泰安271018 [2]山东省泰安市农业科学院,泰安271000

出　　处：《生物技术通报》2006年第3期21-24,共4页Biotechnology Bulletin

基　　金：国家"十五"科技攻关课题[15(种质资源)-002-03];科技部农业科技成果转化资金项目(2004-309)

摘　　要：小麦高分子量谷蛋白亚基(HMW-GS)与小麦品质性状,尤其是沉降值性状显著相关。利用其做分子标记选育聚合优质亚基的品种,具有快速、简单、实用、有效的特点。目前,普通小麦基因组中已有15个已命名Glu-1基因被克隆和测序,这使设计引物序列、进行等位基因的特异性扩增成为可能。对普通小麦高分子量谷蛋白亚基基因组成特点及分子标记现状进行了分析,并针对国内利用高分子量谷蛋白亚基进行分子标记辅助育种做了展望。Wheat HMW-GS is associated with wheat quality characters, especially siguificandy with sedimentation value. The molecular marker of HMW-GS is quick, easy and efficient in convergent breeding. Now, Primer designed and allele specific PCR are applicable because the fifteen genes at Glu-1 site in wheat are cloned and sequence analyzed. The characteristic of HMW genes and their molecular markers are reviewed in this paper. At the same time, the future research prospects on marker assisted selection breeding in our country is discussed.

关 键 词：小麦 高分子量谷蛋白亚基 分子标记 辅助育种 

分 类 号：S512.1[农业科学—作物学]