二元融合子与荧光假单胞菌耐药性遗传标记的筛选  

Selection of Antimicrobial Tolerance Genetic Marker of Protoplast Fusion Between Binary Strain and Pesudomonas fluorescens

作　　者：陈光荣[1] 肖克宇[2] 

机构地区：[1]暨南大学水生所,广州510632 [2]湖南农业大学水产系,长沙410128

出　　处：《生物技术通报》2006年第3期73-76,共4页Biotechnology Bulletin

摘　　要：应用定性和定量药物敏感性试验与诱变相结合,筛选出荧光假单胞菌56-12-10菌株的遗传标记为链霉素抗性,二元融合子AM-1菌株的遗传标记为环丙沙星抗性,确定了在这两种细菌原生质体融合试验中,选择培养基中链霉素的应用浓度为300Iu/ml,环丙沙星应用浓度为100Iu/ml。Using the qualitative and quantitative anfimicrobial susceptibility experiment to screen the genetic markers of protoplast fusion between 56-12-10 strains and AM-1 strains which are Smr （streptomycin resistance） and Cmr （ciprofloxacin resistance）respectively, it can be assured that the applied chroma in selective culture medium of streptomycin and ciprofloxacin are 300 Iu/ml and 100 Iu/ml in the protoplast fusion experiment between two bacteria.

关 键 词：二元融合子 荧光假单胞菌 遗传标记 

分 类 号：S941[农业科学—水产养殖]