降落PCR法快速检测高羊茅转基因植株  

Application of Touch Down PCR in Identifying Tall Fescue Transgenic Plants

作　　者：田路明[1] 黄丛林[1] 张秀海[1] 张潞生[2] 吴忠义[1] 

机构地区：[1]北京农业生物技术研究中心,北京100089 [2]中国农业大学农学与生物技术学院,北京100094

出　　处：《生物技术通报》2006年第3期85-87,共3页Biotechnology Bulletin

基　　金：北京市科委新星计划项目(H020821330130)资助

摘　　要：通过CTAB微量提取高羊茅(Festuca arundinacea)转基因再生植株基因组DNA。用9600型PCR仪器设计降落PCR反应程序,对高羊茅转基因植株两个片段OSISAP1(495bp)和GFP-nos(1 000bp)进行扩增;同时进行了PCR扩增的初步检测。结果表明降落PCR法能快速准确检测转基因植株;而且更适合多个基因片段同时检测,从而提高PCR分子检测的效率,以提高转基因植株鉴定效率。Genomic DNA of transgenic plants of Festuca arundinacea were extracted little by CTAB. The program of touch down PCR was designed in 9600-type PCR machine. Two fragments of OSISAP1 （495bp） and GFP-nos （1 000bp） were amplified with the program designed at the same time and electrophoresis of agar gels shows the products of amplification of two fragments. The results indicated that touch down laCK is efficient in identifying the tall fescue transgenic plants correcdy and quickly and it is fit to amplification of several gene fragments so that it inaproves the efficiency of detection in next experiments.

关 键 词：CTAB 降落PCR 转基因植株 

分 类 号：Q943.2[生物学—植物学]