华贵栉孔扇贝♀×栉孔扇贝♂远缘杂交的受精细胞学观察  

Cytological Observation on Hybrid Fertilization Chlamys nobilis ♀ ×Chlamys farreri ♂ with Fluorescent Microscope

作　　者：王珏[1] 毕克[1] 黄晓婷[1] 张玲玲[1] 胡景杰[1] 包振民[1] 

机构地区：[1]中国海洋大学生命科学与技术学部海洋生物遗传学与种质工程实验室,青岛266003

出　　处：《生物技术通报》2006年第6期122-126,共5页Biotechnology Bulletin

摘　　要：用HOECHST33258对华贵栉孔扇贝(Chlamys nobilis)♀×栉孔扇贝(Chlamys farreri)♂的受精卵进行染色,在荧光显微镜下观察其受精细胞学过程。观察表明:栉孔扇贝的精子能够使华贵栉孔扇贝卵子受精。精子入卵后呈一圆形亮点,体积稍有膨大成球形;授精后成熟卵母细胞释放出第一极体和第二极体后,精核解凝、稀疏、泡状化,形成雄性原核(male pronucleus);雌性原核(female pronucleus)在完成两次成熟分裂之后,染色质去浓缩,扩散膨大;雌雄原核相互靠拢,当雌雄原核膨胀到最大程度时,发生融合,形成合子。受精卵能够正常发育,完成第一次卵裂,卵裂时细胞核分离未观察到异常现象。与华贵栉孔扇贝和栉孔扇贝种内交配对照组相比,异源受精卵的细胞学过程明显滞后,同时其发生过程具明显的不同步现象。实验中还观察到少数的雌核发育现象。In this study,cytological observation with fluorescence microscope was applied for fertilization Chlamys nobilis ♀ ×Chlamys ftareri ♂. Results proved the possibility of hybridization between these two different geographic species. Clearly parts of sperms from C. farreri could bind and penetrate into the mature eggs of C. nobilis which were in prophase or metaphase of meiosis. With the activation from sperms,eggs finished their meiotic division and released polar bodies successively. The male and female pronuclei fused finally. Polar lobes could be found in the first cleavage. Compared with the intraspecific groups,asynchronism and retardance in hybrid development are remarkable. Few eggs developed without the imprint of male nucleus.

关 键 词：华贵栉孔扇贝 栉孔扇贝 杂交 受精 荧光显微观察 

分 类 号：S917.4[农业科学—水产科学]