均匀设计优化超临界CO_2流体萃取大黄蒽醌的研究  

Study on the Uniformity Design Optimization CO_2 Fluid Extract of Pieplant Anthraquinone

作　　者：陈卫林[1] 郭玫[1] 赵磊[1] 余晓晖[1] 杨荣[1] 张梦云[1] 张玉兰[1] 王颖[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《中医药导报》2007年第2期72-74,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:通过均匀设计试验确定超临界二氧化碳流体萃取(SFE-CO2)掌叶大黄中蒽醌类成分的最佳提取工艺条件。方法:以药材粒度、萃取压力、萃取温度、萃取时间和夹带剂用量为考察因素,进行SFE-CO2萃取;用高效液相色谱法测定不同条件下芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量。结果:优选出总蒽醌的最佳萃取工艺条件是萃取压力为38 MPa,萃取温度为70℃,萃取时间为60 m in;夹带剂用量为300 m l。结论:最佳提取工艺可有效萃取掌叶大黄中蒽醌类成分;本含量测定方法可靠、简单易行。Objective： To use the uniformity design test for deciding the best extract technology conditions of pieplant anthraquinone. Method： The inspective factors included granularity of medical material, extractive pressure, extractive temperature and time, dosage of entrainer. SFE - CO2 was used for extracting. HPLC method used to determine the contents of aloe - emodin, chrysophanic acid, archen, chrysophanol, physcione in different conditions. Result： The best extract technology conditions involved that the extrac- tive pressure was 38 MPa, the extractive temperature and time were 70 ℃ and 60 minutes respectively with the dosage of entrainer 300 ml. Conclusion： The best extracting technology can extract the anthraquinone elements in Rheum palmatum L. The method is reliable and simple.

关 键 词：超临界CO2流体 SFE 掌叶大黄 均匀设计 大黄蒽醌 

分 类 号：R927.2[医药卫生—药学]