豚鼠耳蜗血管纹边缘细胞钾离子通道特性的研究  

The Property of Patassium Channel in Marginal Cell of Stria Vascularis of Guinea Pig Cochlea

作　　者：吴婷婷[1] 孔维佳[1] 姚琦 李隽[1] 郭长凯[1] 李之望[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430022 [2]华中科技大学同济医学院神经生物系

出　　处：《听力学及言语疾病杂志》2007年第2期126-128,138,I0001,共5页Journal of Audiology and Speech Pathology

基　　金：国家杰出青年科学基金(39925035);卫生部临床学科重点项目资助(20012332);国家自然科学基金(30070809);高等学校博士学科点专项科研基金(20020487062)资助项目

摘　　要：目的研究单个豚鼠耳蜗血管纹边缘细胞的基本电生理特性。方法应用耳蜗血管纹组织块培养技术和全细胞膜片钳技术,观察单个培养的豚鼠血管纹边缘细胞在电压钳模式下的电流特性。结果边缘细胞上记录到钾离子电流,该钾通道有以下特性:①具有电压依赖性;②通道的活动可被4-氨基吡啶(4-aminopyridine,4-AP)和四乙铵(tetraethylammonium,TEA)在相对高浓度下阻滞;③细胞外钙离子浓度的减少可导致该钾通道电流的降低。结论豚鼠耳蜗血管纹边缘细胞钾离子通道电流包括钙离子依赖性钾电流、外向延迟整流钾电流和A型钾电流。Objective To investigate electrophysiological properties of the patassium channel in the isolated marginal cells of stria vascularis of guinea pig cochlea. Methods The culture technique of cochlear marginal cells of guinea pigs and the whole cell patch - clamp technique were used in this experiment. Results The potassium currents were found in marginal cells of stria vascularis of guinea pig cochlea. The properties of the potassium channel were indecated in that it was voltage- dependent, could be blocked by 4 - AP,TEA in relative high concentration and the currents of this potassium channel contained calcium - dependent potassium current. Conclusion The potassium currents were composed of calcium - activated potassium current, outward delayed rectifier potassium current and A type current.

关 键 词：血管纹 边缘细胞 钾通道 膜片钳 

分 类 号：R339.16[医药卫生—人体生理学]