PACAP对家兔实验性冠状动脉粥样硬化心脏形态重塑的干预作用  

Effects of pituitary adenylate cyclase-activating polypeptide on cardiac remodeling in coronary atherosclerotic rabbits

作　　者：常青[1] 张莉[1] 唐海兰[2] 黄华梅[1] 李自成[3] 

机构地区：[1]暨南大学医学院组织学与胚胎学教研室,广东广州510632 [2]暨南大学实验中心,广东广州510632 [3]暨南大学附属第一医院心内科,广东广州510632

出　　处：《中国病理生理杂志》2007年第3期529-532,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30240076)

摘　　要：目的:研究垂体腺苷酸环化酶激活肽(PACAP)对冠状动脉粥样硬化(AS)形成过程中家兔心脏重塑的干预。方法:雄性新西兰家兔60只,随机等分为正常对照组(C组)、AS模型组(A组)及PACAP干预组(P组)。于实验第4、8、12周末每组随机处死动物5-6只,定位取带有冠脉主干及分支的心脏组织制作石蜡切片,分别行HE、van Gieson染色,在光镜下进行定性观察和/或定量分析。结果:(1)C组心脏组织无病变。A组及P组冠状动脉的主干和小分支内均有斑块形成,心肌间胶原成分增多;但P组冠脉小分支内病变及相应部位胶原沉积较同期A组的轻。(2)A组冠状微动脉重塑指数增大,12周末时(2.58±1.54)显著高于同期C组(1.34±0.58)和P组(1.39±0.48)(P<0.05);心肌细胞最大横径缩小,8周末时(13.85μm±2.27μm)已显著小于同期C组(14.68μm±2.40μm)(P<0.05),12周末时显著小于同期C组和P组。(3)P组的上述相应参数与C组无明显差异(P>0.05)。结论:PACAP可抑制家兔冠状AS形成过程中存在的冠状动脉及心肌形态不良改变。AIM： To study the intervention effects of pituitary adenylate cyclase - activating polypeptide （PACAP） on cardiac remodeling during the development of rabbit coronary atherosclerosis. METHODS： 60 male New Zealand rabbits were equally divided into 3 groups randomly ： control group （ C group）, atherosclerosis model group （ A group） and PACAP intervention group （P group）. At the 4th, 8th and 12th week, 5 -6 cases of rabbits in each group were sacrificed, cardiac tissue with coronary arteries were harvested to make paraffin sections. The sections were stained with hematoxylin - eosin and van Gieson separately. The qualitative observation and/or quantitative analysis were made by light microscope. RESULTS： （1）There was no lesion in C group. For A group and P group, there were plaques in large epicardial coronary arteries and small coronary arteries ; an impressive accumulation of collagen was also observed in myocardium. In P group, the lesions of small coronary arteries were less serious, and the degrees of perivascular and myocardial fibrosis also appeared to be less. （ 2 ） For A group, the wall - to - lumen ratios in small coronary arteries were significant- ly greater at the 12th week （2.58 ± 1.54） than C group （ 1.34 ＋ 0.58） and P group （ 1.39 ± 0. 48） （ P 〈 0.05 ） ; and the width of cardiomyocyte （ 13.85 um ±2. 27 um） was already remarkably narrower than C group （ 14. 68 um ±2. 40 um） at the 8th week （P 〈0. 05） and narrower significantly than C group and P group at the 12th week. （3）There were not difference significantly between the above - related parameters of P group and C group （ P 〉 0. 05 ）. CONCLUSION ： Structure changes exist in coronary arteries and myocardium during the development of rabbit coronary atherosclerosis, PACAP can inhibit the cardiac remodeling.

关 键 词：垂体腺苷酸环化酶激活肽 动脉硬化 心脏 

分 类 号：R544.1[医药卫生—心血管疾病]