VEGF诱导血管内皮细胞产生H_2O_2及其促增殖作用  

VEGF induces HUVECs to produce extracellular H_2O_2 and its proliferation role

作　　者：钱中清[1] 曾耀英[1] 王通[1] 林羿[1] 季煜华[1] 肇静娴[1] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广东广州510632

出　　处：《中国病理生理杂志》2007年第3期533-535,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学重点基金资助项目(No30230305);国家自然科学青年基金资助项目(No30500466)

摘　　要：目的:研究VEGF诱导血管内皮细胞产生细胞外H2O2及其在VEGF促血管内皮细胞增殖功能中的作用。方法:①以H2DCFDA为H2O2指示剂,检测500μg/LVEGF刺激下,血管内皮细胞H2O2的产生;②以MTT法检测3×106U/L过氧化氢酶(CAT),及外源性加入5-20mmol/LH2O2对VEGF促增殖功能的影响。结果:①在VEGF刺激血管内皮细胞15min后,细胞内开始出现逐渐增强的荧光,且随时间逐渐增强,至45min左右最强,随后逐渐减弱;而同时加入过氧化氢酶组的细胞则仅有微弱荧光产生,且荧光强度不随时间变化;②3×106U/L过氧化氢酶对VEGF的促增殖功能有明显的抑制作用(P<0.01);③外源性加入5-10mmol/LH2O2时对血管内皮细胞有明显促增殖作用(P<0.01),但其对VEGF的促增殖功能却有显著抑制作用(P<0.01)。结论:VEGF可刺激血管内皮细胞产生细胞外H2O2,在促细胞增殖中可能具有重要作用。而外源性H2O2对VEGF的生理功能可能具有抑制作用。AIM： To study the effect of VEGF on extracellular H2O2 production in HUVECs and the role of H2O2 in the VEGF- induced proliferation. METHODS： HUVECs was stimulated with 500 ug/L VEGF. Products of extraeellular H2O2 was detected by H2DCFDA staining. MTT method was used to value the influences of 3 × 10^6 U/L eatalase and 5 -20 mmol/L H2O2 to VEGF function. RESULTS： After treatment for 15 min with VEGF, HUVECs appeared fluorescence, and continued to become stronger, peaked at 45 min then decreased. HUVECs, which was treated simultaneity with VEGF and 3 × 10^6 U/L eatalase, only appeared very faint fluorescence. The proliferation of HUVECs by VEGF was restrained when treated with 3 × 10^6 U/L eatalase. The extrinsic H2O2 at concentration of 5 - 10 mmol/L promoted the proliferation of HUVECs but inhibited the proliferation effect of VEGF on HUVECs （ P 〈 0. 01 ）. CONCLUSION ： These findings indicate that VEGF induces HUVECs to produce extraeellular H2O2 and plays role in proliferation, but extrinsic H2O2 restrains VEGF function.

关 键 词：过氧化氢 血管内皮细胞 血管内皮细胞生长因子 过氧化氢酶 

分 类 号：Q255[生物学—细胞生物学]