骨髓源性神经干细胞自体移植海藻酸致痫大鼠海马后脑电图的改变  

EEG changes of the hippocampus after auto-transplantation of bone marrow-derived neural stem ceils into rats with kainic acid-induced epilepsy

作　　者：王焕明[1] 徐如祥[1] 姜晓丹[1] 代广辉[1] 杜谋选[1] 邹雨汐[1] 蔡颖谦[1] 

机构地区：[1]南方医科大学珠江医院神经医学研究所,广州510282

出　　处：《中华神经医学杂志》2007年第3期257-260,共4页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30270491、30400464）、广东省自然科学基金[粤科基办2004（08）]、广东省科技计划项目[粤财企（2001）367、（2003）209、粤科计字（2004）112].

摘　　要：目的探讨骨髓源性神经干细胞移植至海藻酸（KA）致痫大鼠海马后对宿主海马脑电图的影响，从而为神经干细胞移植治疗颞叶癫痫提供理论依据。方法无菌条件下分离大鼠骨髓基质细胞，在特定的条件下培养使其诱导分化为神经干细胞。建立大鼠颞叶癫痫模型，将诱导分化的神经干细胞自体移植至KA大鼠的右侧海马内，观察移植后1周、2周、4周、8周和16周大鼠海马的MRI和脑电图改变情况。结果MRI检查发现在神经干细胞移植后1周和2周时低信号改变区比较局限，但移植4周、8周和16周后低信号改变明显增大，且随着时间的推移低信号改变区逐渐增大。与未移植组相比，移植组大鼠海马脑电图的波幅明显降低，且随着时间的推移降低幅度也明显增加，最高至移植8周后脑电图波幅降低达40％以上。结论骨髓源性神经干细胞自体移植至KA大鼠后对宿主海马的癫痫样放电有一定的抑制作用。Objective To explore the EEG changes after the auto-transplantation of bone marrow-derived neural stem cells （BMSCs-D-NSCs） into the hippocampus of rats with kainic acid （KA） -induced epilepsy, and to provide the theoretical basis of neural stem cells （NSCs） for the treatment of temporal lobe epilepsy （TLE）. Methods Under sterile condition, the bone marrow stromal cells （BMSCs） of rats were isolated, and under specific condition the BMSCs were cultured to induce their differentiation into NSCs. Then the rat models of TLE were established, and the NSCs were auto-transplanted into the right hippocampus of the epileptic rats. The EEG and MRI changes of the hippocampus were analyzed 1, 2, 4, 8, 16 weeks after the transplantation respectively. Results MRI testing showed that low signal region was a little localized 1 and 2 weeks after the transplantation, and then it spread more widely, and with the time going on the low signal change slowly increased 4, 8, 16 weeks after the transplantation. Compared with the non-graft group, the EEG amplitude of the graft group decreasad significantly, and it continued to decrease over time, to the lowest, by more than 40% in 8 weeks after the transplantation. Conclusion When the BMSCs-D-NSCs are transplanted into the KA-induced epileptic rats, they can suppress the epileptogenic discharge of the hippocampus.

关 键 词：骨髓基质细胞 神经干细胞 自体移植 海马 脑电图 

分 类 号：R686[医药卫生—骨科学]