9L／Wistar大鼠脑胶质肉瘤模型的建立  

Establishment of 9L/Wistar rats glioma models

作　　者：杨丽平[1] 赵敬湘[2] 王蕴芳[2] 白慈贤[2] 袁红丰[2] 施双双[2] 闫舫[2] 李露斯[1] 裴雪涛[2] 

机构地区：[1]第三军医大学西南医院神经内科,重庆400038 [2]军事医学科学院输血研究所干细胞与再生医学研究室,北京100850

出　　处：《中华神经医学杂志》2007年第3期270-273,共4页Chinese Journal of Neuromedicine

摘　　要：目的建立稳定的9L／Wistar大鼠脑胶质肉瘤模型。方法构建稳定表达萤火虫荧光素酶PGL的9L细胞株9L^lvc，采用立体定向手术，在Wistar大鼠右侧尾状核接种DAPI荧光标记的9L^lvc细胞，分别于接种后第7，14，21天通过Xenogen活体动物体内成像系统检测肿瘤的生长情况，并断头取材，观察肿瘤的形态学变化，采用免疫组化的方法检测肿瘤细胞胶质纤维酸性蛋白（GFAP）、波形蛋白（Vemintin）的表达。结果大鼠接种9L^lvc细胞后成瘤率100％。活体成像显示细胞接种后第7天到第21天肿瘤呈逐渐增大趋势。组织切片结果显示肿瘤与周围脑组织边界较清楚，未见包膜；瘤内新生血管丰富，可见出血。肿瘤组织免疫组织化学GFAP及Vemintin蛋白染色阴性。结论该方法建立的大鼠脑胶质肉瘤模型稳定可靠，符合恶性胶质肉瘤的生物学特征．是理想的脑胶质肉瘤实验研究材料。Objective To establish stable models of brain tumors by intracerebral inoculation of 9L glioma cells in Wistar rats. Methods The 9L^lvc gliosarcoma cells marked with DAPI were injected stereotactically into the right caudate nucleus in Wistar rats. The characteristics of tumor was detected by Xenogen in vivo at day 7, 14, 21 after implantation. The rats were then decapitated for observing the morphology of tumor by histochemistry and detecting the expressions of glial fibrillary acidic protein （GFAP）, Vemintin by immunohistochemistry. Results The 9L/Wistar rats glioma models were successfully established in all the rats. Tumors showed progressive growth from the 7th to the 21 st day in 9L^lvc/Wistar rats glioma models. Macroscopically the tumor was well demarcated from the adjacent brain; by light microscopy the tumor exhibited malignant characteristics as well as extensive infiltration of the brain parenchyma. Immunoperoxidase GFAP and Vemintin protein were negatively stained in tumor cells. Conclusion Wistar rats bearing 9L gliomas possess the histopathological features of human glioma, which can be used as a stable and reliable model to evaluate various therapeutic approaches.

关 键 词：9L细胞 WISTAR大鼠 脑胶质肉瘤 动物模型 活体检测 

分 类 号：R686[医药卫生—骨科学]