汉坦病毒A9株M基因片段核苷酸序列的测定及分析  

Nucleotide sequencing and analysis of M genome segment of Hantaan virus Chinese isolate A9

作　　者：邱建明[1] 石晓宏[1] 杭长寿[1] R.M.ELLIot 宋干 

机构地区：[1]中国预防医学科学院病毒学研究所,北京100052 [2]英国格拉斯哥大学病毒学研究所

出　　处：《中华微生物学和免疫学杂志》1996年第6期390-394,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家863高技术计划资助

摘　　要：采用反转录-ＰＣＲ方法，分节段扩增汉坦病毒Ａ９株Ｍ基因片段ｃＤＮＡ，并克隆入ｐＧＥＭＴ载体中，用双脱氧末端终止法直接测定序列。结果表明，Ａ９株Ｍ片段ｃＤＮＡ由３６１８个碱基组成，编码１１３５个氨基酸，　　核苷酸序列及由其推导的氨基酸序列同７６／１１８株的同源性分别为９４．３３％和９７．８０％，说明汉坦病毒Ａ９株与７６／１１８株在糖蛋白的结构上高度保守。同７６／１１８株比较，在３'末端非编码区变异极大，变异率为２０％，且多了２个核苷酸。Ａ９株Ｍ基因片段核苷酸序列的获得，为利用该ｃＤＮＡ进行基因工程疫苗的研制创造了条件。The M RNA segment of Hantaan virus Chinese isolate A9 was reverse-transcribed into the first strand of cDNA and amplified into cDNA fragments which were cloned into pGEM-T vector. The positive clones were sequenced on double strand level by dideoxy chain termination method . The M genome segment is 3618 nucleotides in length with a predicated region of 3405 bases encoding a precursor glycopro- tein of 1135 amino acids. An isolate comparison between A9 and 76/118 revealed 94.33％ and 97.80％ homologies at nucleotide and the deduced amino acid sequence level , respectively. Comparing with 76/118, the M genome segment of A9 varied 20％ at 3｀ noncoding region with addition of 2 nucleotides. This demonstrates that the molecular characterization of the glycoprotein of A9 and 76/l18 is highly conservative. Also ,the sequence data provided a fundamental information for the development and application of genetic engineer vaccine against Hantavirus using native M gene cDNA.

关 键 词：汉坦病毒 M基因片段 序列分析 核苷酸 

分 类 号：R373.32[医药卫生—病原生物学]