菌细胞脂肪酸气相色谱图鉴别非发酵菌和肠杆菌  

Gas chromatographic profiles of cellular fatty acids for the identification of Pseudotmonas spp. and Enterobacteriaceae

作　　者：卢焱[1] 叶萍[1] 王靖[1] 张永生[1] 凌代文[1] 翟振兴[2] 戴富保 

机构地区：[1]第二军医大学长海医院,上海200433 [2]第二军医大学药学院

出　　处：《中华微生物学和免疫学杂志》1996年第6期402-404,共3页Chinese Journal of Microbiology and Immunology

摘　　要：本研究用高效毛细管柱气相色谱（ＧＣ）法分析６属１２种１１８株常见非发酵菌和１１种４０株肠杆菌的全细胞脂肪酸（ＣＦＡｓ），并与Ｖｉｔｅｋ－ＡＭＳ的分析结果进行比较。结果表明：两类菌有鉴别意义的ＣＦＡｓ是葵酸、月桂烯酸、月桂酸、十三碳烯酸、十三碳酸、肉豆蔻脑酸、肉豆蔻酸、十五碳烯酸、十五碳酸、十七碳烯酸、十七碳酸、亚油酸、硬脂酸、花生四烯酸、花生酸和二十一碳酸。非发酵菌和肠杆菌的ＣＦＡ构成差别较大。各菌属间的差别较种间大。同属不同种间的差别小且主要是ＣＦＡｓ百分含量不同。ＧＣ法将为临床微生物学检验提供一种全新而高效的检测方法。The cellular fatty acid profiles of 118 strains belonging to 12 different species of nonfermen- tativegram-negative bacteria (NFGNB)and 40 strains to 11 different species of Enterobacteriaceae(EB)were determined by the fused-silica capillary column gaschromatography (GC ) equipped with computer interface and flame ionization detector. The results of GC were compared with the one of Auto Microbic System AMS (Vitek , U. S. A ) analysis. The distinctive patterns of major fatty acids of NFGNB and EB include Capric, Lauroleic, Lauric, Tridecenoic, Tridecanoic, Myristoleic, Myristic, Pentadecenoic, Pentadecanoic, Heptadecenoic , Heptadecanoic , Linoleic , Stearic , Arachidonic , Arachidic and Heneicosanoic acid. In this study , analysis of the CFAs has proven useful for species characterization and identification.In comparing with traditional identification system ,GC is an accurate , simple ,efficient and relatively rapid method for the identification of some strains of bacteria. It will provide an alternative to conventional methods and act as a tool for identification of clinical isolates of bacteria. .

关 键 词：非发酵菌 肠杆菌 气相色谱 细胞脂肪酸 

分 类 号：R378.2[医药卫生—病原生物学]