HLA-DQA1,DQB1基因与特发性膜性肾病关联研究  

Study on HLA-DQA1 , DQB1 genes associated with idiopathic membranous nephropathy

作　　者：张薇[1] 陈楠[2] 董德长[2] 费虹明[3] 蒋伟宏[3] 

机构地区：[1]海第二医科大学附属瑞金医院肾内科张薇,200025 [2]上海第二医科大学附属瑞金医院肾内科张薇,200025 [3]上海第二医科大学生物学教研室

出　　处：《中华微生物学和免疫学杂志》1996年第6期435-437,共3页Chinese Journal of Microbiology and Immunology

摘　　要：为探讨上海地区特发性膜性肾病（ＩＭＮ）与ＨＬＡ－Ⅱ类基因的关联，本研究用聚合酶链反应（ＰＣＲ）及序列特异性寡核苷酸（ＳＳO）探针杂交方法，对３３例上海地区汉族ＩＭＮ患者及７０例同一地区 正常人作了ＨＬＡ－ＤＱＡ１、－ＤＱＢ１位点的等位基因分型，并比较其基因频率。结果表明：ＩＭＮ组ＤＯＡ１０１０１等位基因频率高于对照组，统计学处理有显著性差异（ＲＲ＝３．０４３，Ｐ＝０．００２，Ｐｃ－０．０１８）。ＤＱＢ１０６０４等位基因频率较对照组高，而ＤＱＢ１０３０１等位基因频率较对照组低，但统计学处理后差异均不显著（Ｐ＜０．０５，Ｐｃ＞０．０５），以上结果提示，１ＭＮ的发病机制可能与免疫遗传因素有关。Thirty-three patients with idiopathic memebranous nephyropathy (IMN) in Shanghai Chinese Han were analysed for HLA-DQA1,-DQB1 allele polymorphism by using the PCR technique and sequence specific oligonucleotide (PCR-SSO) probes , 70 controls were also assayed at the same time. A highly significant increased frecquency of the DQA 1* 0101 was found in IMN patients, when compared with control(Pc<0.05). In addition ,an increased frequency of DQH1 * 0604 ,and a decreased frequency of DQB1 * 030l were found , but this was statistically not significant when compared with control (Pc>0.05). These results suggest that the HLA1-DQA1 * 0101 allele was associated with the susceptibility to IMN in Shanghai Chinese Han.

关 键 词：膜性肾病 人类白细胞抗原 病理学 

分 类 号：R692.02[医药卫生—泌尿科学]