LFA-1/ICAM-1单抗对ConA诱导脾淋巴细胞增殖及分泌细胞因子的影响  

Effects of monoclonal antibody of LFA-1 /ICAM- 1 on the Con A induced proliferation and cytokine secretion of splenic lyrnphocytes

作　　者：陈国友[1] 曹雪涛[1] 陶群[1] 马施华[1] 周宏[1] 叶天星[1] 

机构地区：[1]第二军医大学免疫学教研室,上海200433

出　　处：《中华微生物学和免疫学杂志》1996年第4期259-262,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金优秀中青年人才专项基金!(39421009)

摘　　要：通过研究ＬＦＡ－１／ＩＣＡＭ－１单抗对ＣｏｎＡ诱导的脾淋巴细胞活化增殖及其分泌细胞因子的影响　，探　讨了ＬＦＡ－１／ＩＣＡＭ－１分子在Ｔ细胞活化过程中所起的共刺激作用。结果表明，单独应用ＬＦＡ－１α链单抗（Ｍ１７／４．４．１１．９）或ＩＣＡＭ－１单抗（ＹＮ１／１．７．４）均不能引起脾淋巴细胞的增殖，但在加入ＣｏｎＡ诱导脾淋巴细胞增殖反应的最初８小时内加入ＬＦＡ－１单抗可以剂量依赖性地抑制ＣｏｎＡ诱导的脾淋巴细胞增殖反应及脾淋巴细胞分泌ＩＬ－２、ＩＰＮ－γ，而加入ＩＣＡＭ－１单抗却无此效应。说明ＬＰＡ－１在ＣｏｎＡ诱导的Ｔ细胞活化过程中起着重要的共刺激作用，ＬＰＡ－１通过与除ＩＣＡＭ－１以外的其它配体分子（如ＩＣＡＭ－３）相互识别，提供Ｔ细胞活化所必需的共刺激信号。In order to further elucidate the role of LFA- I /ICAM -1 adhesion molecules on T cell activation , we investigated the effects of monoclonal antibody against LFA-1/ICAM - 1 on the Con A induced pruliferation and IL-2 ,IFN-r secretiun of splenic lymphocytes. Our results demonstrated that anti-LFA-1/ ICAM - I alone could not induced splenic lymphocyte proliferation. Adminstration of McAb of LPA- 1 in the first 8h after incubation showed a dose-dependent inhibitory effect on the Con A induced proliferation of splenic lymphocytes while McA b of ICAM- I showed no inhibitory effect. Anti-LFA-l cou M also inhibit Con A induced IL-2 ,IFN -Y secretion from splenic lymphocytes. These results suggested that LFA- 1 could exert its costimulatory function during Con A induced T cell activation through recognition with other receptor molecules other than ICAM- 1.

关 键 词：粘附分子 淋巴细胞 细胞因子 单克隆抗体 

分 类 号：R392.12[医药卫生—免疫学]