小鼠胸腺上皮细胞系MTEC1诱导裸鼠骨髓细胞发生细胞凋亡  

Bone marrow cell apoptosis induced by mouse thymic epithelial cell lioe MTEC 1 in the nude mouse

作　　者：张平夏[1] 余青[1] 戴燕[1] 陈慰峰[1] 

机构地区：[1]北京医科大学免疫学系,100083

出　　处：《中华微生物学和免疫学杂志》1996年第4期293-298,共6页Chinese Journal of Microbiology and Immunology

基　　金：自然科学基金;CMB基金

摘　　要：细胞凋亡（Ａｐｏｐｔｏｓｉｓ）发生在正常生理状态下的分化发育过程中，为胸腺内阴性选择的机制之一。本文报道分别将小鼠胸腺上皮细胞系ＭＴＥＣ１及其培养上清与裸鼠骨髓细胞进行共育，以染色体ＤＮＡ发生规律性断裂为指标，对共育后的裸鼠骨髓细胞的凋亡状况进行分析，结果显示与ＭＴＥＣ１细胞共育后８小时，裸鼠骨髓细胞已发生凋亡，经细胞表型分析，凋亡的细胞可能为粒细胞和Ｂ细胞；而于ＤＭＥＭ中培养８小时以及与ＭＴＥＣ１上清共育８小时的裸鼠骨髓细胞末发生凋亡。结果表明ＭＴＥＣ１可主要通过细胞。细胞间相互作用诱导裸鼠骨髓细胞发生细胞凋亡，凋亡细胞可能主要为粒细胞和Ｂ细胞。Apoptosis takes place under normal physiological condition. lt is most often found during normal tissue turnover, embryogenesis, metamorphosis , and endocrine dependent tissue atrophy. The inference has been made that cell death under these condition is programmed that it is a normal kinetic function of the cell, tissue ,or organ. Apoptosis is one of mechanism in the thymic negative selection. Here we reported that the mouse thymic epithelial cell line MTEC 1 that induced differentiation of nude mouse bone marrow cells in vitro could also mediate nude mouse bone marrow cell apoptosis during 8 hours of cocultivation. Thymocytes could be spontaneously fragmented (no treatment ) and occured during 8 hours of culture in vitro.

关 键 词：细胞凋亡 胸腺基质细胞 骨髓细胞 

分 类 号：R392.12[医药卫生—免疫学]