我国蚕豆根瘤菌的多样性和系统发育研究  

Diversity and Phylogeny of Rhizobia Isolated From the Nodules of Broad Bean (Vicia faba L.) in China

作　　者：路敏琦[1] 李俊[1] 姜昕[1] 李力[1] 沈德龙[1] 曹凤明[1] 

机构地区：[1]中国农业科学院农业资源与农业区划研究所/农业部植物营养与养分循环重点实验室,北京100081

出　　处：《应用与环境生物学报》2007年第1期73-77,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家973项目(001CB1089);国家863项目(2003AA241150)资助~~

摘　　要：采用数值分类、16S rDNA PCR-RFLP、IGS PCR-RFLP等方法对分离自我国11个省的50株蚕豆根瘤菌及11株参比菌株进行了表型测定和遗传型研究,同时对5株蚕豆根瘤菌的代表菌株进行了16S rDNA全序列测定.表型测定的结果表明,在80%的相似水平上供试菌株分为4个群,各群间存在地区交叉;16S rDNA PCR-RFLP的聚类结果与数值分类的聚类结果有很好的一致性;IGSRFLP反映的多样性更明显,形成的遗传群较多,可用于菌株间的鉴别.实验结果表明我国蚕豆根瘤菌具有极大的表型多样性和遗传多样性.系统发育研究结果表明,蚕豆根瘤菌的代表菌株均位于快生根瘤菌属(Rhizobium)系统发育分支,与R.leguminosarum USDA2370的全序列相似性达99.9%,说明蚕豆根瘤菌属于Rhizobium,系豌豆根瘤菌的一个生物型.Fifty Rhizobium strains, isolated from root nodules of broad bean （ Vicaia faba L. ） in 11 provinces of China were studied by numerical taxonomy, 16S rDNA PCR - RFLP, IGS PCR - RFLP and 16S rDNA full length sequence. The 50 strains were divided into four distinctive clusters at the similarity of 80% according to the numerical analysis. The result of 16S rDNA PCR- RFLP showed good agreement with that of numerical taxonomy. Compared with 16S rDNA PCR -RFLP, the result of IGS RFLP showed more differences among the strains. All the results demonstrated a high phenotypic and phylogenetic diversity of Rhizobium sp. （ V. faba L. ）. The full-length sequences of 16S rDNA of 5 representative strains were compared with those of some known rhizobia, and the result displayed that all the representative strains belonged to the same phylogenetic branch of Rhizobium. Fig 4, Tab 3, Ref 12 .

关 键 词：蚕豆根瘤菌 数值分类 遗传多样性 系统发育 

分 类 号：S154.3[农业科学—土壤学]