共振散射光谱法研究人血清白蛋白与二甲酚橙的反应  

Resonance Light Scattering Spectrometric Study on the Reaction of Human Serum Albumin with Xylenol Orange

作　　者：曾铭[1] 李树伟[2] 王小红[2] 

机构地区：[1]花都出入境检验检疫局,广州510800 [2]四川师范大学化学学院,成都610066

出　　处：《理化检验（化学分册）》2007年第2期93-95,101,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：对二甲酚橙作为光散射探针测定蛋白质进行了研究,基于人血清白蛋白对二甲酚橙的共振光散射强度的增强效应,建立了测定蛋白质的共振散射光谱法.在pH4.00的NaOAc-HOAc缓冲溶液中,二甲酚橙只有微弱的光散射强度,但它与蛋白质的缔合物却有较强的共振光散射信号,在λ=517nm处,光散射强度达到最大,并且与蛋白质浓度在为0.0～10.0mg·L^-1范围呈线性,检出限为0.044 mg·L^-1.运用摩尔比法测定二甲酚橙和蛋白质的结合数为132,用于实际样品的分析,测定值与考马斯亮蓝法的结果一致,分析结果的RSD值（n=5）均小于5%.Based on the enhancive effect on the intensity of resonance light scattering （RLS） of xylenol orange （XO） by the presence of human serum albumin （HSA）, a RLS method for the determination of HSA was proposed. The weak resonance scattering light of XO produced in an acetate buffer of pH 4. 00 was remarkably enhanced by its association reaction with HSA having its light intensity maxima at 517 nm. Linear relationship between the light intensity and the concentration of HSA was found in the range of 0. 0- 10.0 mg · L^-1 , with a detection limit of 0. 044 mg · L^-1. Combination ratio of XO to HSA was found to be 132 by the mol ratio method. The proposed method has been used in the analysis of several HSA samples, giving results in conformity with the CBB method, and values of RSD＇s （n=5） less than 5%.

关 键 词：共振光散射 二甲酚橙 人血清白蛋白 人血清白蛋白测定 

分 类 号：O657.31[理学—分析化学]