高效液相色谱法测定灭蟑膏中氯蜱硫磷  

HPLC Determination of Chlorpyrifos in Cockroach Killing Ointment

作　　者：刘绍从[1] 尚为[2] 吕刚[2] 刘军[2] 马军[2] 

机构地区：[1]天津大学化工学院,天津300072 [2]天津出入境检验检疫局,天津300042

出　　处：《理化检验（化学分册）》2007年第2期96-97,101,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：建立了灭蟑膏中1%氯蜱硫磷的超声萃取前处理方法和高效液相色谱分析方法.考查了萃取温度和萃取时间对萃取量的影响.采用μBondapak C18（150 mm×3.9mm,5μm）色谱柱;以乙腈、水、冰乙酸的体积比为82∶17.5∶0.5为流动相;流速1.0 mL·min-1;检测波长280 nm.相对标准偏差为1.8%,回收率在97%～105%之间.HPLC was applied to the determination of chlorpyrifos （-1%） ＂cockroach killing ointment＂ with a pretreatment of the sample by ultrasonic extraction. The effect of temperature and time on the rate of extraction were studied. The μ-Bondapak C18 （150 mm×3. 9 ram, 5 μm） was used as chromatographic column, and a mixed solution containing acetonitrile, acetic acid and water in the ratio of 82 to 0. 5 to 17.5 （by volume） was used as the mobile phase, passing through the column with a flow-rate of 1.0 mL ·min^-1 , UV-detection at the wavelength of 280 nm was used in the measurements. Tests for precision and recovery were made, and the values of RSD （n=6） obtained was 1. 8% and of recovery in the range of 97%- 105%, which were comparable with the gas chromatographic method.

关 键 词：高效液相色谱 灭蟑膏 氯蜱硫磷 超声萃取 

分 类 号：O657.37[理学—分析化学]