洱海沉积物硫循环相关微生物群落结构  

MICROBIAL COMMUNITY COMPOSITION OF SULFUR CYCLE IN LAKE ERHAI SEDI-MENTS

作　　者：王明义[1,2] 梁小兵[1] 郑娅萍[2] 魏中青[1] 赵由之[1] 

机构地区：[1]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550002 [2]贵阳医学院生物化学与分子生物学教研室

出　　处：《现代预防医学》2007年第4期704-705,709,共3页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(40473050;40173038)

摘　　要：[目的]分析洱海沉积物硫循环相关微生物群落多样性和空间分布特点。[方法]倾注平板计数法分析洱海沉积物硫酸盐还原菌和硫氧化细菌数量;PCR分析硫酸盐还原菌类群。[结果]洱海沉积物硫酸盐还原菌和硫氧化细菌数量的空间分布规律相一致;沉积物中检出3个硫酸盐还原菌类群,脱硫叶菌属广泛分布于各层,脱硫肠菌属分布于深层而脱硫球菌-脱硫线菌-脱硫八叠菌属则分布于较浅层。[结论]洱海沉积物硫循环相关微生物群落组成复杂。[Objective] To analyze the diversity and spatial characteristic of microbial community of sulfur cycle in Lake Erhai sedimehts. [Methods] Dumping culture was performed to count the numbers of sulfate-reducing bacteria and sulfate-oxidizing bacteria in the sediments of Lake Erhai, and PCR was utilized to analyze sulfate-reducing bacteria subgroups. [Results] The spatial distribution of sulfate-reducing bacteria coincided with sulfate-oxidizing bacteria. Three sulfate-reducing bacteria subgroups were detected. Desulfobulbus distributed widely, Desulfotomaculum scattered in the deeper sediments and Desulfococcus- Desulfonema - Desulfosarcina spreaded in the lower sediments. [ Conclusion] The microbial community composition of sulfur cycle in Lake Ehai sediments is complex.

关 键 词：沉积物 硫循环 硫酸盐还原菌 硫氧化细菌 

分 类 号：R37[医药卫生—病原生物学]