编码脑组织Nav1.5钠通道新外显子的克隆、鉴定和分布  

Cloning, Distribution and Analysis of The New Exon Encoding Nav1.5 Channel in Brain

作　　者：王军[1] 宗志红[2] 欧绍武[1] 王运杰[1] 任成涛[1] 林毅[1] 孟晓娜[2] 

机构地区：[1]中国医科大学附属一院神经外科,沈阳110001 [2]中国医科大学生物化学与分子生物学教研室,沈阳110001

出　　处：《生物化学与生物物理进展》2007年第3期255-259,共5页Progress In Biochemistry and Biophysics

基　　金：辽宁省教育厅资助项目(05L500)~~

摘　　要：Nav1.5电压-门控钠通道(VGSC)被认为是心肌的特异性通道,但最近的研究发现,该通道在脑组织尤其是边缘系统中亦广泛分布.此前,在对人神经母细胞瘤细胞钠通道的基因克隆中,发现Nav1.5/SCN5A基因的第6A外显子参与编码该通道.采用人及鼠脑组织,通过RT-PCR法对Nav1.5钠通道基因进行克隆发现:Nav1.5/SCN5A基因中的第6A外显子参与编码了该通道,而心肌等其他组织却是第6外显子参与编码该通道.人Nav1.5/SCN5A基因的第6A和第6外显子都定位于3号染色体,共有92个碱基,都可以编码产生30个氨基酸,但却有7个氨基酸不同.人和鼠脑组织Nav1.5/SCN5A基因的第6A外显子仅有一个碱基不同,却产生相同的氨基酸序列.RT-PCR法证实第6A外显子在鼠脑的不同部位表达不同,第6外显子在大鼠不同组织中的表达也不同,这为深入研究不同系统中Nav1.5钠通道的功能提供了基础.Among the tetrodotoxin-resistant（TTX-R） ec-subunits, Nav1.5 mRNA showed the strongest expression in the heart, according with its consideration as the cardiac Na^＋ channel. Compared to its mRNA transcription in the heart, the Nav1.5/SCNSA gene was also expressed in high levels in the brain, especially limbic regions of the brain. Exon6A ofNav1.5/SCN5A gene was first found in cloning ofNav1.5 channel in human neuroblastoma cells. It was found that it is exon6A that encodes Nav1.5 channels in human and rat brain while it is exon6 that encodes Navl.5 channels in other tissues in the cloning ofNav1.5 channel in these tissues, Both exon6A and exon6 have 92 base pairs, which encodes 30 amino acid residues, and are located in human chromosome 3. But these two resulting products differ by 7 amino acid residues. There is only one different base pair between the enon6As encoding Navl.5 channels in human and rat brain, but their encoding amino acid residues are identical. The expressions ofexon6A in different part of the brain are different and the similar result was found in the expressions of exon6 among different tissues of rat when detected by using RT-PCR method. The study is useful for making further investigation in the functional analysis of Nav1.5 channels in different tissues.

关 键 词：第6A外显子 第6外显子 基因克隆 Nav1.5钠通道 脑组织 

分 类 号：Q71[生物学—分子生物学]