补肾壮阳胶囊人参皂苷的含量测定  

Content Determination for Ginsenoside of Invigorate the Kidney Yang Capsule

作　　者：邓晓文 王淑美[2] 冯素香 刘毅林 胡亚楠 高淑娟 

机构地区：[1]盐城市药品检验所,江苏盐城224000 [2]北京中医药大学,博士研究生北京100102 [3]无锡正东药业有限公司,江苏无锡214091

出　　处：《河南中医学院学报》2007年第2期32-34,共3页Journal of Henan University of Chinese Medicine

摘　　要：目的:建立补肾壮阳胶囊的HPLC含量测定方法。方法:采用RP-HPLC法,色谱柱:大连依利特ODSC18(250mm×4.6mm,5μm);流动相:乙进-0.1%磷酸水(80∶20);流速:1.0ml/min;检测波长:254nm;柱温:30℃。结果:人参皂苷Rg1加样回收率为99.72%,RSD%=1.18%;人参皂苷Re加样回收率为99.26%,RSD%=1.66%;人参皂苷Rb1加样回收率为99.89%,RSD%=1.35%。结论:所建立的含量测定方法简便可行、重复性好,可用来评价补肾壮阳胶囊的质量。Objective： To establish RP- HPLC method for content determination of Invigorate the Kidney Yang Capsule. Methods： Using BDS C18 column （200 mm×4.6 mm,5 μm） of Dalian Elite, the mobile phase was a mixture of acetonitrile-water gradient elution. The flow rate was 1.0 ml/min, the wavelength was 203 nm. Results： The average recovery of Ginsenoside Rgl was 99.72% with RSD= 1.18%, The average recovery of Ginsenoside Re was 99.26% with RSD= 1.66%, and the average recovery of Ginsenoside Rbl was 99.89% with RSD= 1.35%. Conclusion： The method is simple, accurate and can be used to for quality supervisorv of Invigorate the Kidney Yang Capsule.

关 键 词：补肾壮阳胶囊 人参皂苷RGL 人参皂苷RE 人参皂苷RB1 HPLC 含量测定 

分 类 号：R286.0[医药卫生—中药学]