高碘对大鼠胸腺上皮细胞影响的超微结构观察  

Ultrastructural observations on the effect of excessive iodine on thymic epithelial cells

作　　者：张国权[1] 吕少文[1] 李红[1] 刘皓[2] 杨海贤[3] 董亚利[3] 

机构地区：[1]武警医学院组胚教研室,天津300162 [2]天津医科大学组胚教研室,天津300070 [3]天津医科大学电镜室,天津300070

出　　处：《武警医学院学报》2007年第2期106-109,F0002,共5页Acta Academiae Medicinae CPAPF

基　　金：武警医学院院级科研基金项目(WY2006-18)

摘　　要：【目的】研究摄入高浓度的碘化钾后,Wistar大鼠胸腺上皮细胞超微结构的变化。【方法】取断乳后1个月的Wistar大鼠,分为5组:适碘组(NI)、5倍碘组(5HI)、10倍碘组(10HI)、50倍碘组(50HI)和100倍碘组(100HI)。饲养3个月后,测定血清甲状腺激素水平,电镜观察胸腺上皮细胞的超微结构。【结果】与NI相比,10HI和50HI的FT3显著降低(P<0.05);100HI甲状腺激素的各项指标均显著降低(P<0.01)。电镜下高碘大鼠胸腺上皮细胞发生了明显变化:张力丝明显增多;溶酶体样致密颗粒明显增多;胞质空泡化;胞浆内出现许多低电子密度的囊泡;胞膜降解。尤其在100HI组,胸腺上皮细胞开始出现损伤性变化。【结论】不同部位的胸腺上皮细胞对甲状腺激素的反应不同;长期摄入过量的碘可使甲状腺激素减少,从而损伤胸腺上皮细胞。[Objective] To study the ultrastructural changes of thymic epithelial ceils （TEC） in condition of excessive iodine. [Methods] Wistar rats, 1 month after weanling, were divided into five groups, normal iodine group （NI）, fivefold high iodine group （5HI）, ten-fold high iodine group （10HI）, fifty-fold high iodine group （50HI） and one hundred- fold high iodine group （100HI）. Three months later, thyroid hormone was measured, thymic epithelial ceils were observed by electron microscopy. [Results] The FT3 level of serum thyroid hormone in 10HI and 50HI groups were lower than that in NI （ P 〈0.05）; the levels of TT4, FT4, TT3, FT3, rT3 in 100HI decreased greatly （ P 〈0.01）. Significant changes as significantly increased tonof＇daments, numerous dense lysosome-like granules, many low electron density vacuoles degeneration of cell membrane and vacuolization could be seen in thymic epithelial cells by electron microscopy. [Conclusions] TECs of different location react to thyroid hormone differently. Excessive intake of iodine can destroy thymic epithelial cells by decreasing thyroid hormones.

关 键 词：碘 大鼠 胸腺上皮细胞 超微结构 

分 类 号：Q25[生物学—细胞生物学]