Luminol-H_2O_2化学发光法测定司帕沙星  

Chemiluminesence Determination of Sparfloxacin by Flow Injection Analysis

作　　者：石杰[1] 曹丰璞[1] 郭玮[1] 陈锦辉[1] 张宗培[1] 

机构地区：[1]郑州大学化学系,郑州450001

出　　处：《郑州大学学报（理学版）》2007年第1期84-87,共4页Journal of Zhengzhou University:Natural Science Edition

基　　金：河南省科技攻关项目;编号0496430006;河南省教育厅自然科学基金资助项目;编号2004150001

摘　　要：基于在碱性介质中，司帕沙星对Luminol—H2O2化学发光体系有较强的增敏作用，建立了司帕沙星的流动注射化学发光分析法．结果表明：司帕沙星浓度在9．0×10^-12～7．6×10^-8mol／L范围内，线性关系良好，检出限为4．8×10^-12mol／L（n=11）．本方法快捷、简便，且具有很高的灵敏度，用于司帕沙星片剂中司帕沙星含量的测定，回收率在96．8％～114．5％之间，结果满意．A new flow injection chemiluminescence method for the determination of sparfloxacin is described. It is found that the chemiluminescence reaction of Luminol-H2O2 can be sensitized by sparfloxacin . On this basis, a flow injection chemiluminescence detection is developed for the determination of sparfloxacin. The linear range is 9.0 × 10^-12 -7.6 × 10^-8 mol/L, and the detection limit is 4.8 × 10^-12 mol/L. The method is easy and rapid with high sensitivity. The method is applied to the determination of sparfloxacin in tablets with satisfactory results

关 键 词：司帕沙星 化学发光 流动注射 

分 类 号：O657[理学—分析化学]