两种条件培养基促进鼠胚胎干细胞分化为造血干细胞(英文)  

Two conditional media promoting the differentiation murine embryonic stem cells into hematopoietic stem cells

作　　者：赵惠萍[1] 卢光琇[1] 王绮如[1,2] 

机构地区：[1]中南大学生殖与干细胞工程研究所,人类干细胞国家工程研究中心,长沙410078 [2]中南大学湘雅医学院血液生理研究室

出　　处：《中南大学学报（医学版）》2007年第1期63-68,共6页Journal of Central South University ：Medical Science

基　　金：This work was supported by National Natural Science Foundation of China (30300119 and30030070) ;Research Fundfor the Doctoral Program of Higher Education from the Ministry of Education of China (20030533002)

摘　　要：目的:从鼠胚胎干细胞获得具有造血重建功能的造血干细胞。方法:将ES-D3细胞形成4天拟胚体(4dEBs),再用骨髓内皮细胞条件培养液(mBMEC-CM)和/或胎肝基质细胞条件培养液(FLSC-CM)诱导4dEBs生成造血干细胞。实验为mBMEC-CM+FLSC-CM组、mBMEC-CM组、FLSC-CM组,对照组。检测诱导生成的细胞造血干细胞特异抗原表达、造血相关基因表达、造血集落的形成以及造血重建能力。结果:从诱导ES-D3细胞生成造血干/祖细胞的数量和生成的集落总数看,mBMEC-CM+FLSC-CM组诱导效率显著高于其他3组。mBMEC-CM+FLSC-CM组诱导生成的细胞能重建照射鼠的造血系统。结论:骨髓内皮细胞条件培养液联合胎肝基质细胞条件培养液可促进鼠胚胎干细胞分化为具有造血重建能力的造血干细胞。Objective To derive hematopoietic stem cells with functional properties of hematopoietic reconstitution from murine embryonic stem （ES） cells. Methods ES-D3 cells by formation of the day-4 embryoid bodies （4dEBs） were induced into hematopoietic stem cells by co-culture with murine bone marrow endothelial cell-conditional medium （mBMEC-CM） and the fetal liver stromal cell-conditional medium （FLSC-CM）. This experiment was designed to 4 groups （ mBMEC-CM ＋ FLSC-CM group, mBMEC-CM group, FLSC-CM group, and the control group）. Results The total cell numbers, CD34 ^＋ cell numbers, and colony numbers formed in the mBMEC-CM ＋ FLSC-CM group were the highest among the 4 groups. The cells in the mBMEC-CM ＋ FLSC-CM group resumed the hematopoietic system of the mice after being transplanted with the inducing cells. Conclusion The culture condition combing mBMEC-CM with FLSC-CM can promote murine ES cells differentiating into hematopoietic stem cells with functional properties of hematopoietic reconstitution.

关 键 词：胚胎 干细胞 拟胚体 造血 造血千细胞 骨髓 内皮细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]