外周血单个核细胞TRAIL mRNA和血清sTRAIL水平与HBV感染肝损伤的相关性  

Involvement of tumor necrosis factor-related apoptosisinducing ligand mRNA in peripheral blood mononuclear cells and serum soluble tumor necrosis factor-related apoptosis-inducing ligand in liver injury caused by chronic hepatitis B

作　　者：毛丽萍[1] 王惠民[2] 张子玉[1] 吴月平[1] 章幼奕[1] 鞠少卿[2] 王陆军[1] 陈育凤[1] 

机构地区：[1]南通大学附属南通第三医院检验科,江苏省南通市226006 [2]南通大学附属医院检验科,江苏省南通市226001

出　　处：《世界华人消化杂志》2007年第6期641-645,共5页World Chinese Journal of Digestology

基　　金：南通市社会发展基金资助课题.No.S2006043~~

摘　　要：目的:探讨慢性乙型肝炎(CHB)患者外周血单个核细胞(PBMCs)肿瘤坏死因子相关凋亡诱导配体(TRAIL)mRNA及血清可溶性TRAIL(sTRAIL)水平与HBV感染肝损伤的关系.方法:采用TaqMan实时荧光定量RT-PCR(FQ- RT-PCR)检测58例慢性肝炎患者外周血PBMCs中TRAIL mRNA水平,采用夹心酶联免疫吸附法分析其血清sTRAIL水平,同时与血清HBV DNA滴度和肝功能相关指标,进行相关性分析.结果:除轻度慢性乙肝外,其他各型慢性乙型肝炎患者血清sTRAIL水平均显著低于健康对照组(t=2.91,P<0.05),各型慢性乙肝之间无显著性差异,与白蛋白(ALB)呈显著正相关(r =0.426,P<0.05),与TBIL、ALT及HBV DNA滴度无相关性.各型慢性乙肝患者PBMCs中TRAIL mRNA水平显著高于健康对照组(t= 28.31.P<0.001),与肝功能指标及血清HBV DNA滴度无相关性.结论:血清sTRAIL水平降低与肝损伤相关,提示单个核细胞膜结合型TRAIL增高与肝损害及乙肝病程迁延不愈有关.AIM： To explore the correlations of tumor necrosis factor-related apoptosis-inducing ligand （TRAIL） mRNA in peripheral blood mononuclear cells （PBMCs） and serum soluble TRAIL （sTRAIL） in liver injury during chronic hepatitis B （CHB）. METHODS： Real-time fluorescence quantitative polymerase chain reaction （FQ-RT-PCR） and sandwich enzyme-linked immunoadsorbent assay were used to detect the expression of TRAIL mRNA in the PBMCs and the level of sTRAIL in serum of 58 CHB patients, respectively. Then correlation analysis was performed between the results and serum HBV DNA titer, albumin, alanine aminotransferase （ALT） and total bilirubin （TBIL） levels. RESULTS： The serum level of sTRAIL in patients with CHB （except for the light type） was significantly lower than that in the healthy controls （t = 2.91, P 〈 0.05）, but there were no significantly differences among patients with various types of CHB. The level of sTRAIL was positively correlated with serum albumin （r = 0.426, P 〈 0.05）, but it had no relationship with HBV DNA titer, TBIL and ALT. The expression of TRAIL mRNA in PBMCs was obviously higher in all the CHB patients than that in the healthy controls （t = 28.31, P〈0.001）, and it had no marked relationship with liver function and HBV DNA titer. CONCLUSION： Lower level of serum sTRAIL may be correlated with CHB-caused liver injury, which indicates that up-regulation of TRAIL expression on the membranes of PBMCs is involvement in the delayed course of hepatitis B.

关 键 词：荧光定量PCR 外周血单个核细胞 肿瘤坏死因子相关凋亡诱导配体 慢性乙型肝炎 

分 类 号：R512.62[医药卫生—内科学]