镇肝熄风汤对自发性高血压大鼠脑保护作用的研究  

Brain protective effect of Zhenganxifeng Decoction in rats with essential hypertension

作　　者：孟云辉[1] 凃欣[2] 吴艳霞[3] 凃晋文(指导) 

机构地区：[1]湖北中医学院,湖北430061 [2]华中科技大学人类基因组研究中心,生命科学与技术学院 [3]华中科技大学同济医学院附属中西医结合医院

出　　处：《北京中医药大学学报》2007年第2期101-104,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2003CB517104);湖北省武汉市卫生局中西医结合科研课题资助项目(No.2004-241)

摘　　要：目的观察镇肝熄风汤对自发性高血压大鼠(SHR)的降压效果及其对血浆、脑组织中内皮素(ET)含量,脑组织中过氧化物酶体增殖物激活受体γ(PPARγ)mRNA表达的影响。方法将24只14周龄的雄性SHR随机分为SHR对照组、镇肝熄风汤组、依那普利组,同时以同源雄性WKY大鼠8只作为对照组。灌胃给药8周后,用16导生理记录系统记录血压,放射免疫法测血浆、脑组织中ET含量,逆转录-聚合酶链反应(RT-PCR)测脑组织中PPARγ mRNA表达。结果与WKY组相比,SHR血压升高,且脑组织中ET含量增加,PPARγ mRNA表达减弱。镇肝熄风汤可使SHR脑组织中ET含量减少,PPARγ mRNA表达增强。结论镇肝熄风汤可能通过激活PPARγmRNA表达,降低SHR脑中ET含量,从而保护脑组织。Objective To observe the depressor effect of Zhenganxifeng Decoction in rats with essential hypertension and the influence on brain endothelin （ET） content and the expression of peroxisome proliferating activator receptor γ （PPARγ） mRNA. Methods All 24 male rats with essential hypertension （14 weeks old ） were randomly divided into the essential hypertension control group, Zhenganxifeng Decoction group, enalapril group, and other 8 male WKY rats were taken as the WKY control group at the same time. The blood pressure was recorded by the sixteen leads physiologic record system, ET contents in serum and brain were detected by radioimmunoassay, and brain PPARγ mRNA expression was detected by reverse transcription - polymerase chain reaction （RT-PCR） after 8 weeks. Results The blood pressure and brain ET content were increased and PPARγ mRNA expression was decreased in the essential hypertension control group compared with those in the WKY control group. Zhenganxifeng Decoction reduced the ET content and improved PPARγ mRNA expression in rats with essential hypertension. Conclusion Zhenganxifeng Decoction protects brain by activating PPARγ mRNA expression and reducing brain ET content.

关 键 词：镇肝熄风汤 自发性高血压大鼠 内皮素 过氧化物酶体增殖物激活受体Γ 

分 类 号：R285.5[医药卫生—中药学]