大黄煎剂对急性肝衰竭大鼠肝性脑病防治机制的实验研究  

The Experimental Study on the Mechanism of Rheum Offcinale Decoction on Hepatic Ence phalopathy in Rats with Acute Hepatic Failure

作　　者：邱华[1] 毛德文[1] 韦艾凌[1] 

机构地区：[1]广西中医学院第一附属医院,南宁530023

出　　处：《中国中医急症》2007年第2期195-197,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：广西壮族自治区自然科学基金项目(No.0542103)

摘　　要：目的观察大黄煎剂对急性肝衰竭大鼠肝性脑病的影响,并探讨其作用机制。方法用皮下注射TAA造成大鼠实验性暴发性肝衰竭。将大鼠60只随机分为空白组、模型组、大黄煎剂组和乳果糖组;造模前3日开始灌胃给药。测定各组大鼠血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、血氨(NH3)、内毒素(LPS)水平,肠内容物ph值,肝线粒体肿胀度及线粒体内单氨氧化酶(MAO)、谷胱甘肽-S-转移酶(GSH-ST)、过氧化氢酶(CAT)活力。结果大黄煎剂组血清AST、ALT、NH3、LPS水平显著降低,肠内容物pH值下降,肝线粒体对外加钙离子引发肿胀的敏感性及MAO、GSH-ST、CAT的活力增强,与模型组比较有显著差异。结论大黄煎剂对肝性脑病具有防治作用,其机制可能是通过抑制肠道内细菌的繁殖,减少肠内氨的生成;通过泻下作用,缩短NH3、LPS等毒素在体内聚集、停留的时间;降低肠道pH,抑制肠道对NH3的吸收;保护肝线粒体的结构和功能,促进NH3的代谢。Objective： To investigate the influence of Rheum Offcianle Decoction on the Hepatic encephalopathy in rats with actue hepatic failure, and to explore the mechanism. Methods： The acute hepatic failure rat models were induced by injection thioacetamide （TAA）. 60 rats were divided into 4 groups randomly： the blank group, the modle group, the rheum officinale decoction group and the lactulose group. Administration of gastric infusion was executed 3 days before model - making. The leves of AST, ALT, NH3, LPS in serum, the PH of caecum content, the swelling and action of MAO, GSH - ST, CAT in hepatic mitochondria were detected. Results： Rheum Offtcinale Decoction was able to notably reduce the levels of AST, ALT, NH3, LPS in serum and the pH of caecum content; increase the sensitivity of swelling to the exotic Ca^2 ＋ and the action of MAO, GSH - ST, CAT in hepatic mitochondria, compare with the modle group. Conclusions： Rheum Offtcinale Decoction had effect of resisting hepatic encephalopathy, the mechanism might be, reducing the production of NH3 by restrainting the reproduction of bacteria in insestines; shortening the settle time of NH3, LPS by dysentery; preventing the absorption of NH3 by lowing the PH of caecum; speeding the metabolism of NH3 by protecting hepatic mitochondria.

关 键 词：肝性脑病 血氨 肠源性内毒素 肝线粒体 大黄煎剂 

分 类 号：R285.5[医药卫生—中药学]