两种标准铀矿样粉对肺巨噬细胞毒性的体外研究  

STUDY ON TOXICITY OF TWO KINDS OF STANDARD URANIUM ORE DUSTS FOR RABBIT ALVEOLAR MACROPHAGES IN VITRO

作　　者：温晋爱[1] 伦汉清[1] 高增林[1] 韩焕清 常学奇[1] 

机构地区：[1]中国辐射防护研究院

出　　处：《工业卫生与职业病》1996年第6期330-333,共4页Industrial Health and Occupational Diseases

摘　　要：应用兔肺泡巨噬细胞测试系统，选用细胞半数致死浓度（ＬＣ_（５０））、乳酸脱氢酶活性和细胞培养液中的^（５１）Ｃｒ释放量等指标，研究了ＧＢＷ０４１０４（Ｎｏ．４和ＧＢＷ０４１０５（Ｎｏ．５）两种标准铀矿样粉的细胞毒性，并与国产标准石英（Ｑ，ｓ）进行了比较。结果：Ｑ，ｓ、Ｎｏ．５、Ｎｏ．４的ＬＣ_（５０）依次为０．０７２、０．２０１、０．３４４ｍｇ／ｍ１；综合毒性指数（ＣＴＩ）依次为１．００００、０．６０９３、０．３５６６，为定量评价粉尘样品的细胞毒性提供了初步依据。Using cultured alveolar macrophages of rabbit.LC_(50)s of cultured cells were calculated,the activity of LDH and the release of ￣(51) Cr from prelabeled rabbit alveolar macrophages were measured.The cytotoxicities of two kinds of standard uranium ore dusts ( No.4 and 5 ) were studied and compared with Chinese standard quartz(0.3). The results showed that the LC_(50)s of Q.s,No. 5 and No.4 were 0.072mg/m1,0.201mg/m1, and 0.344 mg/ml respectively. Composite toxicity indexes were1.0000, 0.6093 and 0.3566. Through this study, primary method was established for quantitative assessment of cytotoxicity of dust samples.

关 键 词：矽肺 标准铀矿样粉 肺泡巨噬细胞 细胞毒性 

分 类 号：R135.2[医药卫生—劳动卫生]