膜联蛋白B2基因的克隆及其真核表达载体的构建  

Cloning and Construction of Eukaryotic Expression Vector of Annexin B2 Gene

作　　者：王义会[1] 闫强[1] 王平[1] 王凯慧[2] 陈明勇[1] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]第二军医大学医学遗传学教研室,上海200433

出　　处：《动物医学进展》2007年第3期31-33,共3页Progress In Veterinary Medicine

摘　　要：根据GenBank已登录的猪囊尾蚴膜联蛋白B2基因序列,设计合成1对特异性引物,应用反转录-聚合酶链反应(RT-PCR)技术从猪囊尾蚴中扩增出膜联蛋白B2基因,将其克隆至pcDNA3.1表达载体上,经酶切鉴定和基因测序表明,目的基因AnnexinB2已正确地整合至表达质粒中,成功构建了膜联蛋白B2基因的真核表达载体。According to the published sequence of annexin B2 gene of Cysticercus cellulosae , a pair of prim ers were designed and synthesized. The annexin B2 gene was amplified by RT-PCR method from Cysticercus cellulosae, and cloned into pcDNA3. 1 vector. The annexin B2 gene was sequenced and compared with the published sequence of annexin B2 gene of Cysticercus cellulosae in the GenBank. The annexin B2 gene was successfully conformed into the expression plasmid. The eukaryotic expression plasmid containing annexin B2 gene was successfully constructed and was helpful for the studies of function of annexin B2.

关 键 词：膜联蛋白B2基因 真核表达载体 克隆 

分 类 号：Q786[生物学—分子生物学]