光质对悬浮培养黄芩细胞生长及黄芩苷积累的影响  

Effects of Light Waves on Cell Growth and Baicalin Accumulation in Suspension Culture of Scutellaria baicalensis Georgi

作　　者：张进杰 徐茂军 周桂飞 

机构地区：[1]浙江工商大学生物工程系,杭州310035

出　　处：《热带亚热带植物学报》2007年第2期135-140,共6页Journal of Tropical and Subtropical Botany

基　　金：浙江省自然科学基金项目(302785)资助

摘　　要：研究了不同光质、光强和光期对悬浮培养黄芩(Scutellaria baicalensis)细胞的生长、细胞中苯丙氨酸氨基裂解酶(PAL)活性及黄芩苷含量的影响。白光、紫外光、红光和蓝光对悬浮培养黄芩细胞PAL活性和黄芩苷含量有不同的影响。白光、紫外光和蓝光对PAL活性和黄芩苷的积累具有诱导促进作用,其中紫外光的促进作用最强,白光和蓝光的促进作用较弱,而红光照射对PAL活性和黄芩苷的积累有一定的抑制作用。在0-60μmolm-2s-1紫外光照射强度范围内,PAL活性和黄芩苷含量随着紫外光照射强度的增加而显著提高。实验结果同时表明,红光照射显著促进黄芩细胞的生长,紫外光抑制黄芩细胞的生长,而蓝光和白光对悬浮培养黄芩细胞生长的促进作用不明显。与黑暗(对照)相比,每天8h光照强度为60μmolm-2s-1的紫外光、16h光照强度为50μmolm-2s-1的红光交替处理,在培养12d后,细胞的鲜重和黄芩苷含量为对照的1.16和3.2倍,黄芩苷的产量达到439mgL-1,是对照的3.8倍。We investigated influences of white light, UV-B, red light and blue light on phenylalanine ammonialyase （PAL） activity, baicalin content and cell growth in suspension culture ofScutellaria baicalensis Georgi. PAL activity and baicalin accumulation were promoted significantly by UV-B, moderately by white and blue lights. Red light had negative effects on PAL activity and baicalin accumulation. Baicalin content and PAL activity remarkably increased with UV-B intensity rising from 0 to 60μmol m^-2s^-1. The cell growth was promoted by red light, inhibited by UV-B, and unaffected by blue and white lights. Following treated with the alternation of 8 h UV-B of 60μmol m^-2s^-1 and 16 h red light of 50μmol m^-2s^-1, fresh cell weight and baicalin content were 1.16- and 3.2-fold, respectively, higher than the controls. The yield of the total baicalin was 439 mg· L^-1 which was 3.8-fold higher than control.

关 键 词：黄芩 悬浮培养 光诱导 PAL 黄芩苷 

分 类 号：S567.239[农业科学—中草药栽培]