重组人白细胞介素11对两种血小板减少症患儿骨髓巨核系祖细胞的作用  

Effect of recombinant human interleukin 11 on bone marrow megakaryocyte progenitors of children with thrombopenia in vitro.

作　　者：白松婷[1] 盛光耀[1] 赵晓明[1] 王璐[1] 邹湘[1] 

机构地区：[1]郑州大学第一附属医院儿科,郑州450052

出　　处：《中华妇幼临床医学杂志（电子版）》2007年第2期71-73,共3页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

摘　　要：目的研究重组人白细胞介素11(recombinant human interleukin11,rhIL-11)对慢性特发性血小板减少性紫癜(chronic idiopathic thrombocytopenic purpura,CITP)与再生障碍性贫血(aplasticanemia,AA)患儿骨髓巨核系祖细胞(megakaryocyte progenitor)体外生长的影响。方法收集17例CITP患儿及14例AA患儿骨髓,对照组为骨髓检查三系造血功能正常、排除血液系统疾病儿童10例。无血清培养各组骨髓单个核细胞(mononuclear cells,MNC),培养体系中包含血小板生成素(TPO)、可溶性补体结合(SCF),IL-3,rhIL-11,或TPO,SCF,IL-3。培养第14天,用流式细胞仪测定CD41+细胞率,CD41结合的碱性磷酸酶抗碱性磷酸酶(alkaline phosphatase-anti-alkalinephos phatase,APAAP)桥联酶标技术鉴定半固体培养的巨核细胞集落形成单位(megakaryocyte colony forming unit,CFU-MK)和巨核细胞爆式集落形成单位(megakaryocyte burst forming unit,BFU-MK)。结果rhIL-11联合TPO等细胞因子,可促进对照组、CITP组CFU-MK和BFU-MK生成(P<0.01),提高CD41+细胞率(P<0.01);但对AA组,CFU-MK和CD41+细胞率无明显的作用(P>0.05)。结论rhIL-11联合TPO等细胞因子,可促进CITP患儿骨髓巨核系祖细胞在体外无血清培养条件下的增殖,提示rhIL-11具有治疗CITP的潜在价值,但对AA患儿的骨髓巨核系祖细胞生成并无明显的效果。Objective To elucidate further the effects of rhIL-11 on bone marrow megakaryocyte progenitors in children with chronic idiopathic thrombocytopenic purpura （CITP） and aplastic anemia （AA） in vitro. Methods Bone marrow mononuclear cells （MNC） from 17 children with CITP （CITP group） and 14 children with AA （AA group） were cultured in serum-free medium with TPO, SCF and IL- 3, rhIL-11 were additionally added or not into the medium. 10 comparably normal children were controls （control group）. The numbers of megakargocyte colony forming unit （CFU-MK）, megakaryocyte burst forming unit （BFU-MK） were measured at 14 days by CD41 linked to a secondary biotinylated antibodyalkaline phosphatase-anti-alkaline phosphatase （APAAP） detection system. CD41^＋ cells were detected by flow cytometer. Results CFU-MK and BFU-MK of CITP group in medium with rhIL-11 cytokine cocktail were higher than those without rhIL-11 （P〈0.01）. Moreover, CD41^＋ cells could be efficiently expanded in medium with the cytokine combination including rhlL-11 in 14 days （P〈0.01）. The same results could be observed in the control group（P〈0.01）. But in the AA group, both of CFU-MK and CD41^＋ cells could not be efficiently expanded with rhIL-11 cytokine cocktail compared with no-rhIL-11 cytokine cocktail （P 〉 0. 05）. Conclusion rhIL-11 can promote the proliferation and maturation of megakaryocyte progenitors and may be used as an effective treatment for CITP. But there was no significance of rhIL-11 on megakaryocyte progenitors in children with AA.

关 键 词：慢性特发性血小板减少性紫癜 再生障碍性贫血 巨核系祖细胞 重组人白细胞介素11 

分 类 号：R725.5[医药卫生—儿科]