早期干预对宫内缺氧缺血大鼠脑电波及存活神经元数目的影响  

Effects of early intervention on electroencephalogram and surviving neurons of infant rats with brain damage.

作　　者：刘玲[1] 陈燕惠[1] 陈达光[1] 

机构地区：[1]福建医科大学附属协和医院儿科,福州350001

出　　处：《中华妇幼临床医学杂志（电子版）》2007年第2期91-93,共3页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

摘　　要：目的探讨早期干预对促进宫内缺氧缺血(HIBD)大鼠脑损伤的修复作用。方法建立HIBD脑损伤大鼠动物模型,随机分为HIBD干预组、HIBD非干预组,以及正常干预组、正常非干预组。干预组采用早期视、触、听觉刺激和丰富环境的早期干预方法(术后24h至生后28d)。而后,采用脑电图检测大鼠脑功能。尼氏染色观察脑组织病理学变化。结果经过干预后,检测大鼠脑电图发现,HIBD干预组背景节律紊乱轻于HIBD非干预组,异常波形出现频率也较HIBD非干预组少见;尼氏染色结果显示,HIBD干预组海马存活神经元数目较HIBD非干预组多,正常干预组存活神经元数目较正常非干预组多。结论通过脑电波,以及存活神经元数目的比较结果表明,早期干预可能促进宫内HIBD大鼠脑损伤的修复。Objective To explore whether early intervention can affect electroencephalogram （EEG） and surviving neurons of infant rats with brain damage or not. Methods A rat model with HIBD in uterus was set up. Animals were divided into four groups （the intervention group treated by HIBD, HIBD-IT; the non-intervention group treated by HIBD, HIBD-NIT; the control with intervention group, Control-IT, the control with non-intervention group, Control-NIT）at random. After 24 h of the operation, the rats of the intervention groups received stimuli of handling, hearing and sight for 14 days, and then they were kept in an enriched environment for 14 day. The rats＇ functional outcome was observed the nerve electricity physiology changes in the central nervous system （CNS） by electroencephalogram. The tapetum histopathology variety was observed in the microscope by the Nissl＇s staining. Results In brain waves of HIBD-IT rats, we observed not only less background rhythm disordered, but also polymorphic slow waves with high amplitude occurred less frequently than that of HIBD-NIT rats, Surviving neurons of the HIBD-IT rats were more than the HIBD-NIT rats by Nissl＇s staining. Conclusion The comparision of EEG and surviving neurons indicated that early intervention might improve the development of infant rats with brain damage.

关 键 词：SD大鼠 缺氧缺血性脑损伤 早期干预 学习记忆 脑电图 

分 类 号：R722.1[医药卫生—儿科]