高压对大麦种子萌发的影响及诱变的DNA分子指纹分析  

Molecular Analysis of High Pressure Induced Barley DNA Structure and Effect of High Pressure on Germination of Barley Seed

作　　者：龙国徽[1] 纪媛[2] 余涛[3] 郭万里[4] 林秀云[4] 刘洋[1] 申斯乐[1] 邹广田[5] 

机构地区：[1]吉林大学生命科学学院,长春130021 [2]吉林大学物理学院,长春130021 [3]吉林农业大学生命科学学院,长春130118 [4]东北师范大学生命科学学院,长春130024 [5]吉林大学超硬材料国家重点实验室,长春130012

出　　处：《吉林大学学报（理学版）》2007年第2期305-310,共6页Journal of Jilin University:Science Edition

基　　金：吉林大学超硬材料国家重点实验室开放课题基金(批准号:2006003)

摘　　要：利用不同静水压力处理萌动的大麦种子,保压2 h,发现高压对大麦种子萌发及幼苗生长均有一定的抑制作用,大麦发芽率和成苗率明显下降,压力达到200 MPa时不再有幼苗成活.高压处理后,幼苗中出现了表型发生变异的植株,选取不同压力处理中表型变异最明显的3棵单株:P1,P2,P3,以ISSR(inter simple sequence repeat)和RAPD(random amplifiedpolymorphic DNA)手段对其进行DNA指纹图谱分析,用选取的21个ISSR引物和19个RAPD引物分别扩增出391和229条带,多态性条带比率(PPB)分别为49.93%和58.59%,在P2和P3中,两种标记分别扩增出112和113多态条带,ISSR检测中原种与P2的平均绝对距离系数为0.136 1,与P3的平均绝对距离系数为0.116 5.RAPD检测中原种与P2的平均绝对距离系数为0.151 3,与P3的平均绝对距离系数为0.195 9.结果显示,原种与P2,P3在ISSR和RAPD的检测中DNA的多态性十分明显.本研究进一步证实,高压是诱导种子植物变异的一种行之有效的新方法.Barley seeds were treated under different high hydrostatic pressures and were kept for 2 h. Compared with those of control, both germination rate and seedling rate decreased considerably with pressure increasing, all barley seeds were dead after treatment under 200 MPa. The above results show that high hydrostatic pressure could affect the seeds germination, seedling growth. Plant growth was inhibited for treated barley during the early growth stage of seedling. The genetic variation of different high hydrostatic pressure treated barley seeds was investigated by RAPD and ISSR analysis, 21 ISSR and 19 RAPD primers generated 391 and 229 bands respectively, of which 49.93% and 58.59% were polymorphic respectively. For P2 and P3, 112 and 113 bands were generated, ISSR analysis indicates that the mean absolute distance coefficient between the original breed and P2 is 0. 136 1, while that between the original breed and P3 is 0.116 5. RAPD analysis demonstrates that the mean absolute distance coefficient between the original breed and P2 is 0. 151 3, while that between the original breed and P3 is 0. 195 9. ISSR and RAPD analyses of these two mutants and the original breeds reveal that there are significantly polymorphism between the two mutants and the original breed.

关 键 词：高静水压 大麦 ISSR RAPD 

分 类 号：Q291[生物学—细胞生物学]