NTG诱变选育埃博霉素高产菌株的研究  

Screening of High Epothilone-producing Mutants of Sorangium cellulosum by NTG Mutation

作　　者：罗立新[1] 陆一鸣[1] 潘力[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510640

出　　处：《现代食品科技》2007年第3期1-3,共3页Modern Food Science and Technology

基　　金：广东省自然科学基金资助项目(031357)

摘　　要：纤维堆囊菌产生的次级代谢产物埃博霉素是受医学界广泛关注的抗癌良药,由于其较低的产量阻碍了其工业化的发展。本文采用纤维堆囊菌DSM11999作为出发菌株,通过NTG化学诱变的方法,诱变浓度300μg/mL,处理时间30min,来筛选优良菌种,最终得到两株埃博霉素A的产量分别为17.3mg/L和17.4mg/L优良菌株。Epothilone is a secondary metabolite of Sorangium cellulosum and a kind of highly effective anti-cancer medicine. However, its yield is very low, which blocks its industrial fermentation production. In this paper, several mutant swains were obtained by NTG mutation with NTG concentration and mutation time being of 300μg/mL and 30min, respectively. Finally two high epothilone A-producing swains were obtained and their yields reached 17.30mg/L and 17.40mg/L,

关 键 词：纤维堆囊菌 NTG 育种 

分 类 号：TQ465[化学工程—制药化工]