p21蛋白在小鼠受精卵不同期的表达及定位  

Expression and localization of p21 protein in different phases of mouse fertilized eggs

作　　者：武迪迪[1] 张盼盼[2] 李雪松[1] 张杰[1] 宗志宏[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生物化学与分子生物学教研室,辽宁沈阳110001 [2]沈阳市第五医院外科

出　　处：《中国医科大学学报》2007年第1期7-8,共2页Journal of China Medical University

基　　金：国家"973"重点基础研究发展规划资助项目(G1999055900-2);国家自然科学基金重点资助项目(39730460)

摘　　要：目的:研究小鼠受精卵不同时期中p21蛋白的细胞表达及定位。方法:根据时间点收集不同时期小鼠卵细胞,应用免疫荧光法检测其p21蛋白的细胞定位。同时用Westernblot法检测p21蛋白的表达。结果:在小鼠受精卵G1期p21蛋白分布在胞浆中,S期向胞核聚集,而G2期则集中分布于细胞核中;不同时期小鼠受精卵中p21蛋白的表达无明显差异。结论:p21蛋白在小鼠受精卵发育中的作用与其细胞定位密切相关。Objective：To investigate expression and location of p21 protein in G1 ,S,and G2 phases of mice fertilized eggs. Methods：The fertilized eggs in G1, S, G2 phases were collected. The expression and location of p21 protein were determined by Western blot and immunofluorescence method,respectively. Results： p21 protein in G1 ,S,and G2 phases of fertilized eggs was located in the cytoplasm,near the nucleus,and in the nuclehs,respectively. No significant difference in p21 expression was found among fertilized eggs in different phases. Conclusion： The location of p21 protein changes in different phases of mouse fertilized eggs.

关 键 词：小鼠受精卵 P21蛋白 细胞周期 定位 

分 类 号：Q132.7[生物学—普通生物学]