嘌呤核苷磷酸化酶活性和外源性透明质酸吸收率对大鼠肝脏缺血再灌注损伤的研究  

Effect of purine nucleoside phosphorylase activity and exogenous hyaluronic acid absorption in rats with liver ischemia/reperfusion injury

作　　者：宋少伟[1] 刘永锋[1] 梁健[1] 何三光[1] 

机构地区：[1]中国医科大学附属第一医院外科,辽宁沈阳110001

出　　处：《中国医科大学学报》2007年第1期17-19,共3页Journal of China Medical University

基　　金：教育部留学回国基金资助项目(教外司留2001[345])

摘　　要：目的:探讨嘌呤核苷磷酸化酶(PNP)活性和外源性透明质酸(HA)吸收率对大鼠肝脏低温保存及常温缺血再灌注损伤的作用。方法:将大鼠肝脏在UW﹑Celsior和HTK3种不同保存液中低温保存16、24h后,用37°CKrebs-Henseleit液连续循环灌注90min,分别于不同灌注时间检测灌洗液中PNP活性和外源性HA吸收率的变化。结果:低温保存16h和24h,UW和Celsior组再灌注60min与15min比较,PNP活性明显增高(P<0.05),而HTK组再灌注30min则显著增高;低温保存16h,UW和Celsior组再灌注60min与15min比较外源性HA吸收率明显下降(P<0.05),而HTK组再灌注30min出现外源性HA吸收率明显下降;低温保存24hUW组再灌注60min外源性HA吸收率明显下降,而Celsior和HTK组30min即出现外源性HA吸收率明显下降。结论:PNP和HA吸收率可作为评价肝脏缺血再灌注损伤的指标。Objective：To explore the effect of pttrine nucleoside phosphorylase （PNP） activity and exogenous hyaluronic acid （HA） absorption after cold storage and normothermic reperfusion in rots with liver ischemia/reperfusion injury, nethods：The rat liver was harvested and preserved in University of Wisconsin （UW）, Celsior, and Hisfidine-Tryptopan-Ketoglutarate （HTK） solutions for 16 or 24 hours,and then reperfused with Krebs-Henseleit solution at 37℃ for 90 minutes. The activity of PNP and the absorption of exogenous HA in the reperfusion solution were determined. Results：After 16-and 24-hour cold storage,compared with 15 minutes after reperfusion,the activity of PNP was significantly higher 60 minutes after reperfusion in UW and Celsior groups, but it significantly increased 30 minutes after reperfusion in group. After 16-hour cold storage,compared with 15 minutes after reperfusion,the absorption of exogenous HA was significantly lower 60 minutes after reperfusion in UW and Celsior groups and significantly decreased 30 minutes after reperfusion in HTK group. After 24-hour cold storage, compared with 15 minutes after reperfusion, the absorption of exogenous HA significantly decreased 60 minutes after reperfusion in UW group but significantly decreased 30 minutes after reperfusion in Celsior and HTK groups. Conclusion：Both PNP activity and HA absorption could be used to evaluate the liver ischemia/reperfusion injury.

关 键 词：嘌呤核苷磷酸化酶 透明质酸 缺血再灌注损伤 大鼠肝脏 

分 类 号：R657.3[医药卫生—外科学]