双嘧达莫预处理对大鼠原位肝移植缺血再灌注损伤的保护作用  

Protective effect of dipyridamole preconditioning on ischemia/reperfusion injury in rat orthotopic liver transplantation

作　　者：张学颖[1] 王伟力[2] 董春鈺 杨志明[3] 

机构地区：[1]中国医科大学附属第四医院消化内科,辽宁沈阳110032 [2]中国医科大学附属第四医院肿瘤放射治疗科 [3]吉林大学附属第一医院小儿外科

出　　处：《中国医科大学学报》2007年第1期27-29,共3页Journal of China Medical University

基　　金：吉林省计委科技攻关基金资助项目(1997-65)

摘　　要：目的:探讨双嘧达莫(DP)预处理对大鼠原位肝移植缺血再灌注损伤(IRI)的保护作用及机制。方法:建立大鼠原位肝移植动物模型,应用不同剂量DP(B组:0.1mg·kg-1、C组:0.2mg·kg-1、D组:0.4mg·kg-1)进行预处理,并与盐水预处理组(A组)进行对照,分别检测移植前、移植后6h血清肝酶谱及移植前、移植后1h及6h肝组织一氧化氮(NO)、髓过氧化物酶(MPO)水平。结果:C、D组与A组比较能明显降低移植后血清肝脏酶学水平、增加组织内NO水平、降低MPO水平(P<0.01),C组作用更为明显。结论:DP预处理能够对大鼠原位肝移植的IRI产生保护作用。Objective： To evaluate the protective effect of dipyridamole （DP） preconditioning on isehemia/reperfusion injury in rat orthotopie liver transplantation and to explore the mechanism. Methods： The rat model of orthotopic liver transplantation was established. The rots were treated with normal saline （group A）,0.1 mg·kg^-1 DP （group B）,0.2 mg·kg^-1 DP （group C）,and 0.4 mg·kg^-1 DP （group D）,respectively. The levels of alanine aminotransferase and aspartate aminotransferase in serum were measured before and 6 hours after transplantation,and the levels of nitric oxide and myeloperoxidase in liver tissue were detected before and 1 and 6 hours after transplantation. Results：Compared with group A,the level of nitric oxide significantly increased and the levels of alanine aminotrasferase,asparatate aminotransferase,and myleoperoxidase significantly decreased in groups C and D （all P 〈 0.01）. Compared with group D,the changes in the levels of the substances mentioned above were more significant in group C. Condusion：DP preconditioning could protect the liver against ischemia/reperfusion injury in rat orthotopic liver transplantation.

关 键 词：双嘧达莫 肝移植 缺血再灌注 

分 类 号：R657.3[医药卫生—外科学]