SARS冠状病毒S蛋白抗原表位串联基因的设计和表达  

Design and expression of antigen epitope of spike protein of severe acute respitary syndrome coronavirus

作　　者：房凯[1] 钟连声[1] 赵雨杰[1] 

机构地区：[1]中国医科大学基础医学院生物信息学教研室,辽宁沈阳110001

出　　处：《中国医科大学学报》2007年第1期33-34,共2页Journal of China Medical University

基　　金：辽宁省科技厅科技计划基金资助项目(2004225009)

摘　　要：目的:表达严重急性呼吸综合征(SARS)冠状病毒S蛋白的抗原表位序列,为研制新型的SARS病毒疫苗提供新思路。方法:应用生物信息学分析软件分析SARS冠状病毒S蛋白的抗原表位序列,设计全新的抗原表位编码基因序列,构建表达载体并在大肠杆菌中表达该基因。结果:设计并合成了SARS冠状病毒S蛋白的抗原表位序列的新基因序列,在大肠杆菌中实现了该基因的高表达。结论:可以重新设计新基因产生新型候选重组疫苗。Objective：To design a new gene encoding the antigen epitope of the spike protein （S protein） of severe acute respiratory syndrome coronavirus （SARS-CoV） and express the new gene. Methods：By analyzing the antigen epitope of SARS-CoV S protein with antigenic pepfides prediction software,a new gene was designed,and its expression vector was constructed. Then the gene was expressed in Escherichia coli （E coli）. Results：The new gene encoding the antigen epitope of SARS-CoV S protein was designed and synthesized successfully,and it was highly expressed in E cob＇. Conclusion：The new gene encoding the antigen epitope of SARS-CoV S protein could be designed, which provides basis for the development of the new type of recombinant vaccine candidate.

关 键 词：严重急性呼吸综合征 抗原表位 生物信息学 S蛋白 

分 类 号：R563.8[医药卫生—呼吸系统]