骨髓瘤独特型抗原负载树突细胞介导的抗肿瘤效应研究  

The study of anti-tumor immune response in vitro mediated by dendritic cells pulsed with myeloma idiotype

作　　者：黄晖蓉[1] 张连生[1] 张玉芳[1] 柴晔[1] 

机构地区：[1]兰州大学第二医院血液肿瘤科,兰州730030

出　　处：《临床血液学杂志》2007年第2期81-83,共3页Journal of Clinical Hematology

摘　　要：目的:研究骨髓瘤独特型抗原(Idiotype,Id)负载树突细胞(DC)对同源细胞因子诱导的杀伤细胞(CIK)体外抗瘤活性的影响。方法:采集健康供者外周血单个核细胞(PBMNC)用常规方法诱导DC和CIK细胞,将骨髓瘤OPM-2细胞培养上清提取的Id冲击或未冲击的DC与CIK细胞共培养(CIK、DC加CIK、Id-DC加CIK),用流式细胞术分析细胞表型,MTT法检测体外效应细胞杀伤活性。结果:在(5～20):1效靶比范围内, CIK细胞对OPM-2和K562细胞的杀伤率分别为(24．47±3．00)％～(40．64±1．62)％和(23．36±1．51)％～(42．52±2．06)％。DC加CIK及Id—DC加CIK对OPM-2和K562细胞的杀伤活性均高于CIK组,差异有统计学意义(P<0．05);而在相同效靶比之下,Id-DC加CIK对OPM-2细胞的杀伤活性最强,差异有统计学意义(P <0．05)。结论:CIK细胞对骨髓瘤细胞有强的杀伤活性,经Id负载的DC与CIK细胞共培养能进一步增强其特异性杀伤活性,对骨髓瘤可能有免疫治疗作用。Objective：To study in vitro killing effect of idiotype-pulsed dendritic cells （DC） co-cultured with autologous cytokine induced killer（CIK） cells. Method：Peripheral blood mononuclear cells （PBMNC） isolated from healthy donors were induced to obtain DC and CIK cells by conventional methods, the DC pulsed with or without idiotype （Id） precipitated from MM cell line OPM-2 culture supernatant was co-cultured with CIK （CIK, DC＋ CIK and Id-DC＋CIK）. Cells phenotypes were analyzed by flow cytometry. The cytotoxic activity was measured by MTT assay. Result.. The cytotoxic activity of CIK cells alone to OPM-2 and K562 cells was （24.47 ± 3.00）%- （40.64±1.62）% and （23.36±1.51）%-（42.52±2.06）% respectively at effector to target ratios of （5-10 ： 1）. In the same conditions, the cytotoxic activities of DC＋CIK and Id-DC＋CIK were higher than CIK cells （ P 〈0.05）. Furthermore Id-DC＋CIK had the maximal cytotoxic activity to OPM-2 cells （ P 〈0. 05）. Conclusion： CIK cells showed high lytic activity against myeloma cells, which could be enhanced by co-culturing with Id-pulsed DC. It is promising as an immuno-therapeutic strategy for MM.

关 键 词：细胞株 树突细胞 细胞因子诱导的杀伤细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]